文献类型：期刊文献

英文题名：Preparation of a light-sensitive and reversible dissolution copolymer and its application in lysozyme purification

作者：Wen, Wen[1]; Wan, Junfen[1]; Cao, Xuejun[1]; Xia, Jinan[2]

机构：[1]E China Univ Sci & Technol, Dept Biochem Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Donghua Univ, Dept Appl Phys, Shanghai 200051, Peoples R China

年份：2007

卷号：23

期号：5

起止页码：1124

外文期刊名：BIOTECHNOLOGY PROGRESS

收录：;EI(收录号：20074310883294);WOS:【SCI-EXPANDED(收录号:WOS:000250010000020)】；

语种：英文

外文关键词：Copolymerization - Dissolution - Enzyme activity - Laser beam effects - Synthesis (chemical)

摘要：A novel light-sensitive and cation-exchange copolymer (P-NBCC) has been synthesized by random copolymerization of chlorophyllin sodium copper salt, crylic acid, n-butyl acrylate, and N-isopropylacrylamide. The PNBCC copolymer showed reversible dissolution and could be precipitated by 488 nm laser irradiation with the least light density of 1.70 x 10(5) W/m(2). By optimizing the ratio of monomers, pH, and ion concentration, over 95% copolymer was recovered by laser irradiation. The copolymer was used to purify lysozyme as light-sensitive cation exchanger, and its adsorption matched a Langmuir adsorption isotherm with maximum adsorption capacity of 98 900 U/g and dissociated constant of 852 U/mL. By applying the copolymer to the separation of lysozyme from egg white, the specific activity of lysozyme was improved from 399 to 6346 U/mg and the recovery of lysozyme achieved 81.3%.