文献类型：期刊文献

英文题名：Enhancement of lovastatin production by supplementing polyketide antibiotics to the submerged culture of Aspergillus terreus

作者：Jia, Zhihua[1,2]; Zhang, Xiaoli[3]; Zhao, Yaling[3]; Cao, Xuejun[2]

机构：[1] College of Life Sciences, Northwest AandF University, 22 Xinong Road, Yangling, Shaanxi 712100, China; [2] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China; [3] College of Chemical Engineering, Northwest University, 229 North Taibai Road, Xi'an 710069, China

年份：2010

卷号：160

期号：7

起止页码：2014

外文期刊名：Applied Biochemistry and Biotechnology

收录：EI(收录号：20101312806268)

语种：英文

外文关键词：Cultivation - Antibiotics - Aspergillus - Biochemistry - Ketones

摘要：Feedback inhibition existed in lovastatin biosynthesis from Aspergillus terreus. Exogenous lovastatin and other different polyketide antibiotics biosynthesized by polyketide synthase were supplemented to the cultures of A. terreus to investigate their influences on lovastatin production. Supplementing exogenous lovastatin of 100 mg l-1 at the early stage of fermentation and the fast stage of its biosynthesis resulted in decreases of 76.4% and 20% in final lovastatin production, respectively. However, the fungal cell growth was not affected; the growing cycle was only prolonged in the submerged cultivation. Separate supplementation of the five kinds of polyketide antibiotics such as tylosin, erythromycin, tetracycline, daunorobin, and rifamycin to the cultures resulted in increases of about 20~25% in the final lovastatin production. Especially, supplementing tylosin of 50 mg l-1 at the beginning of lovastatin biosynthesis led to the final lovastatin production of 952.7±24.3 mg l-1, which was improved by 42% and 22% compared with that produced in the control and the original culture, respectively. These results are helpful to understand the regulations on lovastatin biosynthesis and improve the final desired metabolite contents in many antibiotics production. ? 2009 Humana Press.