文献类型：期刊文献

英文题名：Highly efficient synthesis of ethyl (S)-4-chloro-3-hydroxybutanoate and its derivatives by a robust NADH-dependent reductase from E-coli CCZU-K14

作者：He, Yu-Cai[1,2];Tao, Zhi-Cheng[1];Zhang, Xian[2];Yang, Zhen-Xing[1];Xu, Jian-He[2]

机构：[1]Changzhou Univ, Coll Pharmaceut Engn & Life Sci, Lab Biocatalysis & Bioproc, Changzhou 213164, Peoples R China;[2]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2014

卷号：161

起止页码：461

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20142017717720);WOS:【SCI-EXPANDED(收录号:WOS:000335436000061)】；

基金：All authors gratefully acknowledge support from the National Natural Science Foundation of China (No. 21102011) and the Open Project Program of the State Key Laboratory of Bioreactor Engineering (Shanghai, China).

语种：英文

外文关键词：Asymmetric reduction; Biotransformation; Ethyl 4-chloro-3-oxobutanoate; Ethyl (S)-4-chloro-3-hydroxybutanoate; Recombinant E. coli CCZU-K14

摘要：An NADH-dependent reductase (CmCR) from Candida magnoliae was discovered by genome mining for carbonyl reductases. After CmCR was overexpressed in Escherichia coli BL21, a robust reductase-producing strain, recombinant E. coli CCZU-K14, was employed for the efficient synthesis of ethyl (S)-4-chloro-3-hydroxybutanoate ((S)-CHBE) from the reduction of ethyl 4-chloro-3-oxobutanoate (COBE). After the optimization, the optimum reaction conditions were obtained. Notably, E. coli CCZU-K14 had broad substrate specificity in reducing both aliphatic and aromatic substrates, and excellent enantioselectivity of CCZU-K14 was observed for most of the tested substrates, resulting in chiral alcohols of over 99.9% ee. Moreover, COBE at a high concentration of (3000 mM) could be asymmetrically reduced to (S)-CHBE in the high yield (>99.0%) and high enantiometric excess value (>99.9% ee) after 14 h. Significantly, E. coli CCZU-K14 shows high potential in the industrial production of (S)-CHBE and its derivatives (>99.9% ee). (C) 2014 Elsevier Ltd. All rights reserved.