文献类型：期刊文献

英文题名：Intracellular pH sensing and targeted imaging of lysosome by a galactosyl naphthalimide-piperazine probe

作者：Fu, Youxin;Zhang, Junji[1];Wang, Huan;Chen, Jia-Li;Zhao, Ping;Chen, Guo-Rong;He, Xiao-Peng[1]

机构：[1]East China Univ Sci & Technol, Key Lab Adv Materials, 130 Meilong Rd, Shanghai 200237, Peoples R China; East China Univ Sci & Technol, Inst Fine Chem, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2016

卷号：133

起止页码：372

外文期刊名：DYES AND PIGMENTS

收录：;EI(收录号：20162602536668);WOS:【SCI-EXPANDED(收录号:WOS:000381844500045)】；

基金：This research is supported by the 973 project (2013CB733700), the National Natural Science Foundation of China (21402050, 21420102004), the Programme of Introducing Talents of Discipline to Universities (B16017) and the Shanghai Rising-Star Program (16QA1401400).

语种：英文

外文关键词：Intracellular pH probe; Lysosome imaging; Glycosylation; Naphthalimide; Photo-induced electron transfer

摘要：A series of galactosyl naphthalimide-piperazine derivatives have been synthesized as intracellular pH and lysosome-targeting imaging probes for live human hepatoma cells. The probes show good sensitivity in both aqueous buffer and intracellular environments. Incorporation of the galactose moiety with the pH probes facilitates their specific endocytosis and, thus targeted trafficking to lysosome, by the asialoglycoprotein receptor of human hepatoma cells. The acidic intracellular pH of live human hepatoma cells gives rise to a fluorescence "turn-on" signal of the probes probably via a modulation of the photo induced electron transfer (PET) mechanism. Additionally, galactosylation of the probes enhances their selective accumulation in lysosome and decreases the cytotoxicity. (C) 2016 Elsevier Ltd. All rights reserved.