文献类型：期刊文献

英文题名：Kaempferol-7-O-β-D-glucoside (KG) isolated from Smilax china L. rhizome induces G₂/M phase arrest and apoptosis on HeLa cells in a p53-independent manner

作者：Xu, Wen[1,2];Liu, Jianwen[1,2];Li, Changlong[1,2];Wu, He-Zhen[3];Liu, Yan-Wen[3]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China;[3]Hubei Coll Tradit Chinese Med, Key Lab Resources & Chem Chinese Med, Wuhan 430061, Hubei, Peoples R China

年份：2008

卷号：264

期号：2

起止页码：229

外文期刊名：CANCER LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000256776900008)】；

语种：英文

外文关键词：Smilux china L.; Kaempferol-7-O-beta-D-glucoside; G(2)/M phase arrest; apoptosis; HeLa human cervix carcinoma cells

摘要：Kaempferol-7-O-beta-D-glucoside (KG), a flavonoid glycoside, isolated from Smilax china L. rhizome, displayed marked anticancer activity on a panel of established cancer cells, of which, HeLa human cervix carcinoma cells were the most sensitive. Meanwhile, the cytotoxic effects of KG on normal human cells (HEK293 embryonic kidney cells and L-02 embryonic liver cells) were much smaller than on cancer cells. This work studied the molecular mechanisms underlying KG induced growth inhibition in HeLa cells. The results showed that KG induced G(2)/M phase growth arrest correlated with Cyclin B1 and Cclk1 decrease in a p53-independent manner, and also caused an increase in apoptosis, which was confirmed by characteristic morphological changes, evident DNA fragmentation, increased apoptotic sub-G1 population. Furthermore, inhibition of NF-kappa B nuclear translocation, up-regulation of Bax and down-regulation of Bcl-2, were observed in HeLa cells treated with KG, which indicated that the mitochondrial pathway was involved in the apoptosis signal pathway. In Summary, KG displayed a significant anti-tumor effect through cell cycle arrest and apoptotic induction in HeLa cells, which suggested that KG might have therapeutic potential against cervix carcinoma. (C) 2008 Elsevier Ireland Ltd. All rights reserved.