文献类型：专利

英文题名：New DNA molecule encoding epoxide hydrolase comprising specified nucleotide sequences, useful for hydrolyzing and racemizing epoxide to prepare optical pure chiral intermediate and plant growth, development and resilience

作者：HE W;ZHAO J;XU J;FAN L

机构：[1]UNIV EAST CHINA SCI&TECHNOLOGY

申请号：CN102382847-A

申请日：2010-08-27

公开日：2012-03-21

语种：英文

收录：DERWENT

摘要：NOVELTY - DNA molecule encoding epoxide hydrolase, in which: a nucleotide sequence comprises a sequence of SEQ ID NO: 1 (comprising a fully defined 960 base pair sequence as given in the specification); DNA molecule is hybridized with the DNA sequence under stringent condition and at the strict condition; and a protein sequence derived through the basic group by substitution and/or deletion, has more than 90% of homology with the nucleotide sequence, is new. USE - The DNA molecule is useful: for hydrolyzing and racemizing the epoxide to prepare optical pure chiral intermediate; and for plant growth, development and resilience (all claimed). No biological data given. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: (1) epoxide hydrolase having an amino acid sequence of SEQ ID NO: 2 (comprising a fully defined 319 amino acid sequences as given in the specification) and amino acid sequence through one or more amino acid residues by substitution, deletion and/or addition in the amino acid sequence; (2) a constituting method of reconstructed expression vector for coding the DNA molecule comprising: designing a specific primer based on the nucleotide sequence of the epoxide hydrolase gene and the multiple cloning sites variable for the expression vector, amplifying the target gene in the complementary DNA template; connecting with the corresponding expression vector after the limitative enzyme cutting; converting and sieving to obtain the reconstructed expression plasmid; converting into the corresponding host cells; and expressing in the host cells, where the host cells are prokaryotic cell or eukaryotic cell; (3) the reconstructed expression vector obtained by the above method, comprising the DNA molecule; and (4) a probe molecule, comprising 10-100 consecutive nucleotides complementary sequence in the DNA molecule.