文献类型：期刊文献

英文题名：Dominance of Desulfotignum in sulfate-reducing community in high sulfate production-water of high temperature and corrosive petroleum reservoirs

作者：Li, Xiao-Xiao[1,2];Liu, Jin-Feng[1,2];Yao, Feng[3];Wu, Wei-Lin[3];Yang, Shi-Zhong[1,2];Mbadinga, Serge Maurice[1,2,5];Gu, Ji-Dong[4];Mu, Bo-Zhong[1,2,5]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Inst Appl Chem, Shanghai 200237, Peoples R China;[3]Jiangsu Oilfield, Inst Petr Engn & Technol, Yangzhou 225009, Jiangsu, Peoples R China;[4]Univ Hong Kong, Sch Biol Sci, Pokfulam Rd, Hong Kong, Hong Kong, Peoples R China;[5]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China

年份：2016

卷号：114

起止页码：45

外文期刊名：INTERNATIONAL BIODETERIORATION & BIODEGRADATION

收录：;EI(收录号：20162402481048);WOS:【SCI-EXPANDED(收录号:WOS:000383528000007)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 41273084 and No. 41530318) and the 863 Program (Grant No. 2013AA064403).

语种：英文

外文关键词：Microbial community; Sulfate-reducing prokaryotes (SRP); High temperature petroleum reservoirs; Microbiologically influenced corrosion (MIC); dsr gene; Souring

摘要：Petroleum reservoirs souring and pipelines corrosion are severe in Jiangsu Oilfield of China, and microorganisms have a major role in the related problems. This study revealed the microbial diversity and composition of both bacteria and archaea by using 16S rRNA gene clone library method, and those of sulfate-reducing prokaryotes (SRP) by dissimilatory sulfate reductase alpha-subunit encoding gene (dsr) clone library in two different samples of high temperature petroleum reservoirs containing high concentration of sulfate and with known corrosion. In addition, the abundance of bacteria and SRP in the samples was quantified by real-time PCR assays based on 16S rRNA and dsr genes. The results showed that both production water samples from Wei 2-53 and Wei 11-7 wells exhibited very rich bacterial diversity, and both Gammaproteobacteria and Alphaproteobacteria were the main abundant ones. Thioalkalivibrio-related species belonging to Gammaproteobacteria were also observed in both of them. Methanomicrobia, Archaeoglobi and Thermococci were common archaea in these samples, with Archaeoglobus as the dominant genus in both of them. The dsrA libraries analysis showed that a low diversity of sulfidogenic communities with Desulfotignum spp. as the most dominant SRP in the samples. The data reported here enhance our knowledge on microbial community and abundance including SRP in high temperature and corrosive reservoirs, providing a better basis for further research on and management control of reservoir souring and biocorrosion in oil production systems. (C) 2016 Elsevier Ltd. All rights reserved.