文献类型：期刊文献

英文题名：Inhibitive effect of prodigiosin on the proliferation of human malignant pancreatic cancer cells

作者：Zhang, J; Liu, JW; Shen, YL; Wei, DZ; Li, K

机构：[1]E China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2005

卷号：14

期号：4

起止页码：181

外文期刊名：MEDICINAL CHEMISTRY RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000236528600001)】；

语种：英文

摘要：Pancreatic cancer is not only common, but also extremely difficult to treat, for which it has been called "the challenge of the twenty-first century". In this study, we find that prodigiosin could effectively inhibit the proliferation of human pancreatic cancer cells H8898 in a dose-and-time-dependent manner, with an IC50 of 75 mu mol according to the results of MTT and cell proliferation assays. This inhibitive effect may relate to two factors: mitotic arrest and cell death. Results of clone formation and Flow cytometry analysis (FCAS) suggested that prodigiosin has the capability of restraining mitosis by regulating the cell cycle. Prodigiosin also could induce apoptosis of pancreatic cancer cells at low concentration and results in the fragmentation pattern of DNA. Prodigiosin may effectively enter cells and promote the level of intracellular reactive oxygen species (ROSin) in a dose-dependent manner. The generation of ROS may play an important role in the cytotoxic effect. All these results demonstrate that prodigiosin can obviously inhibit the proliferation of pancreatic cancer cells H8898 by arresting the cell cycle and inducing apoptosis. Increased ROS lead this cytotoxic effect.