文献类型：期刊文献

中文题名：基因组重排提高珍贵橙色束丝放线菌中安丝菌素P-3

英文题名：Genome Shuffling for the Improvement of Ansamitocin P-3 in Actinosynnema pretiosum

作者：李瑞华[1];李娟[1];胡凤仙[1]

机构：[1]华东理工大学生物工程学院,上海200237

年份：2021

卷号：40

期号：9

起止页码：3248

中文期刊名：基因组学与应用生物学

外文期刊名：Genomics and Applied Biology

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：国家自然科学基金青年基金项目(21406070)资助

语种：中文

中文关键词：基因组重排;安丝菌素P-3;原生质体;珍贵橙色束丝放线菌

外文关键词：Genome shuffling;Ansamitocin P-3;Protoplast;Actinosynnema pretiosum

摘要：安丝菌素P-3(ansamitocin P-3,AP-3)是一种具有高抗肿瘤活性的安莎类抗生素,为了进一步提高AP-3高产菌的产量,开展了利用基因组重排技术提高AP-3发酵水平的研究。首先对AP-3生产菌珍贵橙色束丝放线菌(Actinosynnema pretiosum)原生质体制备、处理、融合条件进行了初步优化,然后以常温常压等离子体(atmospheric room temperature plasma,ARTP)诱变技术得到的高产菌株L-40、L-117、L-506、L-522作为出发亲本进行三轮基因组重排,通过高通量筛选最终筛选出一株高产融合子G3-96,其AP-3产量约为335.9 mg/L,该菌株AP-3产量比亲本提高了47.5%,比野生型菌株提高了93.8%。实验结果表明基因组重排对于产物产量的提升是个非常不错的策略,为发酵生产合成提供了良好的菌株基础。
Ansamitoci n P-3(AP-3)which belonging to ansamycins has high antitumor activity.To further improve the AP-3 productivity in Actinomycetes,the technology of genome shuffling was employed.Firstly,the conditions of protoplasts preparation,treatment and fusion of A.pretiosum were optimized.And then the highyielding strains L-40,L-117,L-506 and L-522 mutated by ARTP(atmospheric room temperature plasma)were used as the starting parental strains for genome rearrangement.After three rounds of genome rearrangement,a mutant strain G3-96 was screened by high throughput screening.Its AP-3 yield in shaking flask fermentation reached to 335.9 mg/L,which was 47.5%and 93.8%higher than that of par-ental strain L40 and wild-type strain.The experimental results showed that genome rearrangement was a fine stra-tegy for increasing the yield of production and also provided good chassis strains for the fermentation to produce AP-3.