文献类型：期刊文献

英文题名：Performance characteristics and community analysis of a single-stage partial nitritation, anammox and denitratation (SPANADA) integrated process for treating low C/N ratio wastewater

作者：Zhou, Feng[1];Xiao, Wan-Zi[1];Zhou, Kai-Yuan[1];Zhuang, Jin-Long[1];Zhang, Xu[1];Liu, Yong-Di[1,2];Ni, Bing-Jie[3];Shapleigh, James P.[4];Zhou, Min[5];Luo, Xi-Zi[5];Li, Wei[1,2,6]

机构：[1]East China Univ Sci & Technol, State Environm Protect Key Lab Environm Risk Asse, Sch Resources & Environm Engn, Shanghai, Peoples R China;[2]Shanghai Inst Pollut Control & Ecol Secur, Shanghai, Peoples R China;[3]Univ Technol Sydney, Ctr Technol Water & Wastewater, Sch Civil & Environm Engn, Sydney, NSW, Australia;[4]Cornell Univ, Dept Microbiol, Ithaca, NY USA;[5]Shanghai Chem Ind Pk Sino French Water Dev Co Ltd, Shanghai, Peoples R China;[6]Nanjing Univ, State Key Lab Pollut Control & Resource Reuse, Nanjing, Peoples R China

年份：2022

卷号：433

外文期刊名：CHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20220211438733);WOS:【SCI-EXPANDED(收录号:WOS:000784350700003)】；

基金：Acknowledgements This work was supported by National Natural Science Foundation of China (52170076) , Shanghai Educational Development Foundation (18CG34) , Shanghai Rising-Star Program (20QC1400500) , State Key Laboratory of Pollution Control and Resource Reuse (PCRRF20024) .

语种：英文

外文关键词：One-stage; Partial nitritation; Anammox; Denitratation; Nitrogen flux

摘要：This study describes the development of an air-lift internal circulation reactor that integrates partial nitrification, anammox and denitratation (SPANADA) into a single stage bioprocess for the treatment of low C/N coal gasification wastewater. During 245 days of operation, the compartmental fluidized bed reactor achieved a total inorganic nitrogen (TIN) removal efficiency of 91.4%. Reads-based metatranscriptomic analysis found the expression of the amoA and hao genes essential for nitritation and the hzsA and hdh genes essential for anammox increased dramatically as reactor performance improved and stabilized. Another notable trend was that the total expression of the napA and narG genes, essential for denitratation, was 3-fold higher than the combined reads for nirK and nirS whose products, nitrite reductases, would lower nitrite levels reducing available substrate for anammox. Analysis of metagenome-assembled genomes revealed members of Nitrosomonas and Candidatus Brocadia, were the dominant genera of ammonium-oxidizing bacteria and anammox bacteria, respectively, and accounted for 5.5% and 10.0% of the total reads in the transcriptome. For denitratation, Thiolinea, whose only relevant gene involved in N-metabolism is narG, accounted for 8.5% of the total reads in the transcriptome and, remarkably, 84.1% of narG expression. Mass balance confirmed anammox was the dominant nitrogen removal pathway, accounting for 67.0% of the TIN removed. Nitritation and denitratation accounted for 82.7% and 17.3% of the nitrite production, respectively. The analysis reported demonstrates the development of a novel and effective one-stage nitrogen removal alternative for low C/N wastewater treatment and also helps gain insight into the underlying microbial interactions.