文献类型：期刊文献

英文题名：Precise Construction of Cell-Instructive 3D Microenvironments by Photopatterning a Biodegradable Hydrogel

作者：Cai, Zhengwei[1];Huang, Kaiping[1];Bao, Chunyan[1];Wang, Xuebin[1];Sun, Xiangchao[2];Xia, Hong[2];Lin, Qiuning[1];Yang, Yi[1];Zhu, Linyong[1]

机构：[1]East China Univ Sci & Technol, Optogenet & Synthet Biol Interdisciplinary Res Ct, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Jilin Univ, Coll Elect Sci & Engn, State Key Lab Integrated Optoelect, Changchun 130012, Jilin, Peoples R China

年份：2019

卷号：31

期号：13

起止页码：4710

外文期刊名：CHEMISTRY OF MATERIALS

收录：;EI(收录号：20193307317002);WOS:【SCI-EXPANDED(收录号:WOS:000475408400010)】；

基金：This work was supported by NSFC (grant nos. 21472044 and 21425311). The authors thank the Research Center of Analysis and Test of East China University of Science and Technology for the help on the characterizations.

语种：英文

外文关键词：Biodegradation - Cells - Biomimetics - Cell culture

摘要：On the basis of the complexity of the extracellular matrix, it is very important to control the three-dimensional (3D) biochemical and physical properties of hydrogels at the microscale level. In this study, we demonstrated a synthetic strategy to construct biomimetic hydrogels (HA-MMP) for 3D cell culture that uses thiol-Michael chemistry to encapsulate cells in situ, o-nitrobenzyl alcohol photochemistry (a photogenerated-aldehyde-amine-ligation sequence, which is also referred to as a PAAL sequence) to permit biochemical patterning, and an enzyme-cleavable reaction to facilitate cell-responsive degradation in hydrogels. The effects of biochemical and mechanical parameters on 3D cell culture were studied using a user-programmed Boolean logic-based algorithm, which provided an "AND" logic gate for cell survival, spreading, and migration. Finally, using the spatial controllability of light, cell-instructive 3D microenvironments can be constructed to guide cell behavior in hydrogels. This approach enables the versatile nature of chemistry to create programmable niches in hydrogels, which provides valuable insights into the cell fate by changing the local hydrogel microenvironments.