文献类型：期刊文献

英文题名：Identification and mechanism of skin soothing and barrier-improving components in Broussonetia papyrifera extract

作者：Han, Qifeng[1];Wu, Mengqi[1];Zhu, Mulan[2];Liu, Yuxuan[1];Yang, Xiang[1];Xia, Wei[1];Zhang, Wenqing[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai, Peoples R China;[2]Chinese Acad Sci, Ctr Excellence Mol Plant Sci, Natl Key Lab Plant Mol Genet NKLPMG, Shanghai 200032, Peoples R China

年份：2025

外文期刊名：NATURAL PRODUCT RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001531046600001)】；

语种：英文

外文关键词：Broussonetia papyrifera; anti-inflammatory; skin barrier-improvement; HaCaT/THP-1 co-culture; ceramide lipidomics

摘要：Broussonetia papyrifera is traditionally used in Chinese medicine to treat inflammatory skin disorders. This study screened bioactive fractions from B. papyrifera ethanolic extract with anti-inflammatory and skin barrier-improving effects. A flavonoid-rich fraction (BP-F4) significantly inhibited monocyte chemoattractant protein-1 (MCP-1) and the monocyte activation marker CD86 in an in vitro skin inflammation model (TNF-alpha-induced co-culture of HaCaT keratinocytes and THP-1 monocytes), while an alkaloid fraction (BP-Alkaloids) promoted ceramide production in HaCaT cells as demonstrated by lipidomics. UPLC-QTOF-MS/MS identified 29 flavonoids in BP-F4 and 18 pyrrolidine alkaloids in BP-Alkaloids. RNA-Seq analysis revealed that BP-F4 suppressed the chemokine signalling pathway (HSA04062), whereas BP-Alkaloids activated the PPAR signalling pathway (HSA03320). These findings validate the therapeutic potential of B. papyrifera and provide a rapid screening workflow for skin-active phytochemicals.