文献类型：期刊文献

英文题名：Molecular sieving polymer for DNA/RNA separation in capillary electrophoresis

作者：Liu, Chenchen[1];Yamaguchi, Yoshinori[1,2];Dou, Xiaoming[1,2]

机构：[1]ECUST, Inst Photon & Biomed, Grad Sch Sci, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Osaka Univ, Grad Sch Engn, Dept Appl Phys, 2-1 Yamada Oka, Suita, Osaka 5650871, Japan

年份：2017

卷号：31

期号：16-19

外文期刊名：INTERNATIONAL JOURNAL OF MODERN PHYSICS B

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000406349900093)】；

基金：This work was partly supported by JSPS KAKENHI (Grant No. A15H038270 (Y.Y)).

语种：英文

外文关键词：Molecular sieving polymer; RNA; polyacrylamide; capillary electrophoresis

摘要：In capillary polymer electrophoresis, the property of polymer sieving matrix dominates the migration behavior of DNA/RNA. We investigated the capillary electrophoresis of RNA ranging from 100 nt to 10,000 nt in polyacrylamide (PA) solutions with different molecular weights (Mw) and different concentrations. We observed that the resolution length (RSL) of RNA fragments was improved and the migration time was prolonged, when polymer concentration was increased. The resolution for small RNA fragments (< 1000 nt) was improved with the increase of polymer concentration, whereas the large ones (> 3000 nt) became inseparable. In addition, we estimated the smallest resolvable nucleotide length (Ls) by the plot of RSL against RNA size.