文献类型：期刊文献

英文题名：Anti-CD133 Antibody Immobilized on the Surface of Stents Enhances Endothelialization

作者：Li, Jian[1];Li, Dan[1];Gong, Feirong[2];Jiang, Shaoyan[1];Yu, Hua[1];An, Yi[1]

机构：[1]Qingdao Univ, Affiliated Hosp, Dept Cardiol, Coll Med, Qingdao 266003, Shandong, Peoples R China;[2]E China Univ Sci & Technol, Sch Mat Sci & Engn, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China

年份：2014

卷号：2014

外文期刊名：BIOMED RESEARCH INTERNATIONAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000333309400001)】；

基金：This work was supported by the National Natural Science Foundation of China (81071246 and 81370323); Shandong Provincial Natural Science Foundation, China (ZR2010HM081); and China Postdoctoral Science Foundation (2012M511462).

语种：英文

摘要：Drug eluting stents successfully reduce restenosis at the cost of delayed reendothelialization. In recent years, a novel concept to enhance reendothelialization using anti-CD34 antibody coated stents which capture circulating progenitor cells (EPCs) has been developed with conflicting clinical results. CD133 is a glycoprotein expressed on circulating hematopoietic and putative endothelial-regenerating cells and may be superior to CD34 for EPCs capture stents. In the present study, anti-CD133 antibody has been successfully immobilized to the biodegradable polymeric coating material by covalent conjugation. We explore whether anti-CD133 antibody coated stents (CD133 stents) might accelerate reendothelialization in comparison with bare metal stents (BMS) through the superior ability to capture EPCs. The in vitro cell culture results indicate that anti-CD133 antibody functionalized polymer film significantly promotes CD133 positive cells attachment and growth compared with the unfunctionalized polymer film. In the semi-in vivo arteriovenous shunt model CD133 stents demonstrate much quicker specific capturing of EPCs from the blood stream than BMS within 6 hours. In a porcine coronary artery injury model CD133 stents show more effective reendothelialization in short term compared with BMS, while no significant difference in endothelial function recovery was observed between these two groups within 6-month followup.