文献类型：期刊文献

英文题名：Nanoscale Zr-Based MOFs with Tailorable Size and Introduced Mesopore for Protein Delivery

作者：Wang, Zhe[1];Hu, Shuanggang[2];Yang, Jian[1];Liang, Ajuan[2];Li, Yongsheng[1];Zhuang, Qixin[1];Gu, Jinlou[1]

机构：[1]East China Univ Sci & Technol, Sch Mat Sci & Engn, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Renji Hosp, Shanghai Key Lab Assisted Reprod & Reprod Genet, Sch Med,Ctr Reprod Med, Shanghai 200135, Peoples R China

年份：2018

卷号：28

期号：16

外文期刊名：ADVANCED FUNCTIONAL MATERIALS

收录：;EI(收录号：20180804810407);WOS:【SCI-EXPANDED(收录号:WOS:000430163700030)】；

基金：This work was financially supported by the Natural Science Foundation of China (51072053, 51372084), Science and Technology Commission of Shanghai Municipality (17DZ2271100), and the 111 Project (B14018).

语种：英文

外文关键词：drug delivery; hierarchically porous MOFs; mesoporous UiO-66; MOF nanoparticles; protein encapsulation

摘要：Introduction of large pore in the primitive microporous metal-organic frameworks (MOFs) with tailorable particle size can endow them with desired properties for potential applications in the intracellular delivery of membrane-impermeable proteins. However, no research is found to focus on this topic until now. Herein, a monocarboxylic acid (MA) and organic base comodulation strategy is developed to synthesize the hierarchically porous UiO-66 nanoparticles. MA of dodecanoic acid is utilized to control the pore size while trimethylamine (TEA) plays a key role in modulating the nucleation of crystallization to regulate the particle size. In comparison with microporous UiO-66, a model protein of cytochrome c (Cyt c) could be efficiently loaded into the mesoporous MOFs (mesoMOFs). The size-dependent cellular uptake is also evaluated, and it is verified that mesoMOFs with particle size of 90 nm could be endocytosed into living cells with highest efficiency. These outstanding merits enable the current mesoMOFs not only to exhibit efficient encapsulation of Cyt c but also facilitate the protein delivery into the cytosol and subsequent endosomal escape. Given the exceptional chemical stability, hierarchically porous structure as well as tunable particle size, the elaborated mesoUiO-66 nanoparticles might offer a promising platform for a variety of biomedical applications.