文献类型：期刊文献

英文题名：Omics Application of Bio-Hydrogen Production Through Green Alga Chlamydomonas reinhardtii

作者：Xu, Lili[1];Fan, Jianhua[2,3];Wang, Quanxi[1]

机构：[1]Shanghai Normal Univ, Coll Life Sci, Dept Biol, Shanghai, Peoples R China;[2]Hainan Univ, State Key Lab South China Sea Marine Resource Uti, Haikou, Hainan, Peoples R China;[3]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China

年份：2019

卷号：7

期号：AUG

外文期刊名：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY

收录：;EI(收录号：20193607406717);WOS:【SCI-EXPANDED(收录号:WOS:000482233600001)】；

基金：This work was supported by the National Natural Science Foundation of China 31600284, Shanghai Engineering Research Center of Plant Germplasm Resources 17DZ2252700, Shanghai Pujiang Program (18PJD008), Open Funding Project of the State Key Laboratory of Bioreactor Engineering 2018021, and Open Funding Project of the State Key Laboratory of Marine Resource Utilization in South China Sea (Hainan University) 2019002.

语种：英文

外文关键词：Chlamydomonas reinhardtii; hydrogen production; genomics; transcriptomics; proteomics; metabolomics

摘要：This article summarizes the current knowledge regarding omics approaches, which include genomics, transcriptomics, proteomics and metabolomics, in the context of bio-hydrogen production in Chlamydomonas reinhardtii. In this paper, critical genes (HydA1, Hyd A2, Sulp, Tla1, Sta7, PFL1) involved in H-2 metabolism were identified and analyzed for their function in H-2 accumulation. Furthermore, the advantages of gene microarrays and RNA-seq were compared, as well as their applications in transcriptomic analysis of H-2 production. Moreover, as a useful tool, proteomic analysis could identify different proteins that participate in H-2 metabolism. This review provides fundamental theory and an experimental basis for H-2 production, and further research effort is needed in this field.