文献类型：期刊文献

英文题名：Live-cell imaging reveals redox metabolic reprogramming during zygotic genome activation

作者：Sun, Hao[1,2,3,4];Zhang, Zhuo[5,6];Li, Tianda[1,2,3];Li, Ting[5,6];Chen, Weicai[5,6];Pan, Tianshi[1,7];Fang, Sen[1,2,3,4];Liu, Chao[1,2,3];Zhang, Ying[1,2,3];Wang, Leyun[1,2,3];Feng, Guihai[1,2,3];Li, Wei[1,2,3,4];Zhou, Qi[1,2,3,4,8];Zhao, Yuzheng[5,6,9]

机构：[1]Chinese Acad Sci, Inst Zool, State Key Lab Stem Cell & Reprod Biol, Beijing, Peoples R China;[2]Chinese Acad Sci, Inst Stem Cell & Regenerat Med, Beijing, Peoples R China;[3]Beijing Inst Stem Cell & Regenerat Med, Beijing, Peoples R China;[4]Univ Chinese Acad Sci, Beijing, Peoples R China;[5]East China Univ Sci & Technol, Optogenet & Synthet Biol Interdisciplinary Res Ctr, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Pharm,State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[6]Chinese Acad Med Sci, Res Unit New Tech L Cell Metab Imaging, Beijing, Peoples R China;[7]Northeast Agr Univ, Coll Life Sci, Harbin, Peoples R China;[8]Chinese Acad Sci, Inst Zool, State Key Lab Stem Cell & Reprod Biol, Beijing 100101, Peoples R China;[9]Chinese Acad Med Sci, Res Unit New Tech Live Cell Metab Imaging, Beijing 100730, Peoples R China

年份：2023

卷号：238

期号：9

起止页码：2039

外文期刊名：JOURNAL OF CELLULAR PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001010100600001)】；

基金：National Key Research and Development Program of China, Grant/Award Numbers: 2018YFE0201100, 2019YFA0904800, 2019YFA0110100, 2021YFA0719300, 2022YFA1104300; NSFC, Grant/Award Numbers: 32150030, 32030065, 32121005, 92049304; International Cooperation Project of China Manned Space Program; Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism; Chinese Academy of Medical Sciences, Grant/Award Numbers: 2019RU01, 2019-I2M-5-013; State Key Laboratory of Bioreactor Engineering; Fundamental Research Funds for the Central Universities

语种：英文

外文关键词：biosensor; dynamic imaging; embryo development; metabolite detection; single cell

摘要：Metabolic programming is deeply intertwined with early embryonic development including zygotic genome activation (ZGA), the polarization of zygotic cells, and cell fate commitment. It is crucial to establish a noninvasive imaging technology that spatiotemporally illuminates the cellular metabolism pathways in embryos to track developmental metabolism in situ. In this study, we used two high-quality genetically encoded fluorescent biosensors, SoNar for NADH/NAD(+) and iNap1 for NADPH, to characterize the dynamic regulation of energy metabolism and redox homeostasis during early zygotic cleavage. Our imaging results showed that NADH/NAD(+) levels decreased from the early to the late two-cell stage, whereas the levels of the reducing equivalent NADPH increased. Mechanistically, transcriptome profiling suggested that during the two-cell stage, zygotic cells downregulated the expression of genes involved in glucose uptake and glycolysis, and upregulated the expression of genes for pyruvate metabolism in mitochondria and oxidative phosphorylation, with a decline in the expression of two peroxiredoxin genes, Prdx1 and Prdx2. Collectively, with the establishment of in situ metabolic monitoring technology, our study revealed the programming of redox metabolism during ZGA.