文献类型：期刊文献

中文题名：华东地区某饮用水源地中磺胺类抗性基因的分布特征

英文题名：Distribution Characteristics of Sulfonamide Antibiotic Resistance Genes in a Drinking Water Source in East China

作者：胡亚茹[1];姜蕾[2];张天阳[1];雷丹丹[1];姜巍巍[2];张东[2];林匡飞[1];崔长征[1]

机构：[1]华东理工大学资源与环境工程学院,国家环境保护化工过程环境风险评价与控制重点实验室,上海200237;[2]上海城市水资源开发利用国家工程中心有限公司,上海200082

年份：2018

卷号：39

期号：9

起止页码：4222

中文期刊名：环境科学

外文期刊名：Environmental Science

收录：CSTPCD;;EI(收录号：20184906207368);Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；PubMed;

基金：国家水体污染控制与治理科技重大专项(2017ZX07402003); 中国博士后科学基金项目(2017M621391); 上海市科委科技攻关项目(16DZ1204703); 上海市青年科技启明星计划项目(15QB1400700)

语种：中文

中文关键词：磺胺类抗性基因;I型整合酶基因intI1;饮用水源水;分布特征;水平基因转移

外文关键词：sulfonamides antibiotics resistance genes; integrase gene int11; drinking water source; distribution characteristics;horizontal gene transfer

摘要：饮用水源中检测到的抗生素抗性基因(antibiotic resistance genes,ARGs)对饮用水质安全和人体健康产生的潜在威胁受到广泛关注.在掌握了华东地区某饮用水源地13种磺胺类抗生素的污染特征基础上,进一步采用定性PCR和荧光定量PCR解析该饮用水源水和底泥中磺胺类ARGs(sul1、sul2)以及抗性基因可转移元件Ⅰ型整合酶基因(int I1)的分布特征.结果表明,3种基因在该饮用水源水和底泥中均100%检出,sul1基因是该饮用水源地中检出含量最高的磺胺类ARGs,在水源水中含量范围为1.5×104~6.4×105copies·mL^(-1),底泥中则高达1.6×108copies·g^(-1),较sul2、int I1基因分别高0.6~2.2、0.5~1.9个数量级.sul1、sul2和int I1基因在该水源地入水口和出水口处的绝对含量无显著差别,而在底泥中sul1、sul2和int I1基因的绝对含量则是出水口高于入水口.sul1在夏季水源地出水口的检出含量最高,为6.4×105copies·mL^(-1);int I1基因在冬季的检出含量高于其他季节.sul1基因与13种磺胺类抗生素具有相关性(r=0.69,P<0.05),其中与磺胺甲唑的含量显著相关(r=0.79,P<0.01);int I1与sul1、sul2的相对含量之间也存在正相关关系(r为0.80和0.73,P<0.05),这表明int I1在磺胺类ARGs的水平转移过程中起到了重要作用.本研究为典型饮用水源地中ARGs的污染现状提供基础数据,也为管控饮用水环境的抗性基因污染和制定管理决策提供依据.
Antibiotic resistance genes（ARGs） in drinking water sources have attracted widespread attention due to the threat they pose to water security and human health. This study mainly focused on the distribution of sulfonamide ARGs（sul1,sul2） and one integrase gene（int I1） in water and sediment using qualitative and fluorescent quantitative PCR,based on previous work on the characteristics of13 kinds of sulfonamides in a drinking water source in East China. Results showed that the three target genes were all detected in water and sediment. The sul1 gene was the sulfonamide ARG with highest concentration,with 1. 5 × 10^4-6. 4 × 10^5 copies·mL^-1 in source water and maximum concentration of 1. 6 × 10^5 copies·g^-1 in sediment. Concentration of sul1 was 0. 6-2. 2,0. 5-（-1）. 9 order of magnitudes higher than sul2 and int I1 genes,respectively. There was no significant difference between the absolute concentrations of sul1,sul2,and int I1 in inflow and outflow. However,in the case of sediment,absolute abundances of sul1,sul2,and int I1 in outflow were higher than those in inflow. The maximum concentration of sul1 was detected in outflow in summer（6. 4 × 10^5 copies·mL^-1）. The concentration of int I1 was higher in winter compared to other seasons. There was a positive correlation between sul1 and 13 sulfonamides（r = 0. 69,P〈0. 05）,and the relative concentration of sul1 and amount of sulfamethoxazole were significantly positively related（r = 0. 79,P〈0. 01）. There were also positive correlations between the relative concentrations of int I1 and sul1,sul2（r：0. 80 and 0. 73,P〈0. 05）,respectively,suggesting that int I1 played an important role in horizontal gene transfer of sulfonamide ARGs in this drinking water source. This study provides basic data for monitoring pollution of ARGs,as well as a basis for controlling ARG pollution in the drinking water environment and making management decisions.