文献类型：期刊文献

英文题名：Simultaneous improvement of lincomycin A production and reduction of lincomycin B levels in Streptomyces lincolnensis using a combined medium optimization approach

作者：Zheng, Xiu-hong[1];Ye, Rui-fang[1];Ding, Qi-hang[1];Hu, Feng-xian[1];Zhang, Hong-zhou[2];Lai, Shen[2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Topfond Pharmaceut Co Ltd, Zhumadian 463000, Henan, Peoples R China

年份：2022

卷号：72

期号：1

外文期刊名：ANNALS OF MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000777997000001)】；

语种：英文

外文关键词：Streptomyces lincolnensis; Lincomycin; Medium optimization; Osmotic stress; qRT-PCR

摘要：Purpose The current study aimed to optimize the culture and production parameters of industrial production of lincomycin A by Streptomyces lincolnensis using a statistical approach that could also reduce unwanted by-products. Methods The Plackett-Burman design, steepest ascent method, and response surface design were used to evaluate different factors that affect lincomycin A production. Results Using an optimized S. lincolnensis fermentation medium, lincomycin A production was increased up to 4600 mg/L in shaking flasks, which indicated a 28.3% improvement over previous production in an un-optimized medium (3585 mg/L). Additionally, the concentration of lincomycin B by-product was reduced to 0.8%, which was 82.2% lower than that in the un-optimized medium. Further, quantitative real-time PCR analysis revealed the optimized medium improved lincomycin A production by stimulating key genes in the lincomycin A biosynthesis pathway, as well as an osmotic stress gene. Conclusions Based on the results, the sequential optimization strategy in this study provides powerful means for the enhancement of lincomycin A with less by-product. We found that osmotic stress reduced the concentration of lincomycin B, which could also help reduce fermentation by-product yields in other actinobacteria.