文献类型：期刊文献

英文题名：Spatiotemporal control of gene expression by a light-switchable transgene system

作者：Wang, Xue[1];Chen, Xianjun[1];Yang, Yi[1]

机构：[1]E China Univ Sci & Technol, Synthet Biol & Biotechnol Lab, State Key Lab Bioreactor Engn, Sch Pharm, Shanghai 200237, Peoples R China

年份：2012

卷号：9

期号：3

起止页码：266

外文期刊名：NATURE METHODS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000300890400022)】；

基金：We thank Z.H. Yu and J.Z. Chen for their suggestions, and Z.M. Du, Z.C. Ma, J.H. Wang, W.T. Zhu, X.Y. Feng and Y.Z. Zhao for technical assistance. This work was supported by the National Natural Science Foundation of China (grants 31170815, 31071260 and 90713026), the 863 Program (grant no. 2006AA02Z160), the Fok Ying Tung Education Foundation (grant 111022), the Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning, Dawn Program of Shanghai Education Commission (grant 11SG31), Doctoral Fund of Ministry of Education of China (grant 20100074110010), the Fundamental Research Funds for the Central Universities and the 111 Project (grant B07023).

语种：英文

摘要：We developed a light-switchable transgene system based on a synthetic, genetically encoded light-switchable transactivator. The transactivator binds promoters upon blue-light exposure and rapidly initiates transcription of target transgenes in mammalian cells and in mice. This transgene system provides a robust and convenient way to spatiotemporally control gene expression and can be used to manipulate many biological processes in living systems with minimal perturbation.