文献类型：期刊文献

英文题名：The glucose RQ-feedback control leading to improved erythromycin production by a recombinant strain Saccharopolyspora erythraea ZL1004 and its scale-up to 372-m3 fermenter

作者：Chen, Yong[1,2];Wang, Zejiang[1];Chu, Ju[1];Xi, Beili[3];Zhuang, Yingping[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Qingdao Agr Univ, Food Sci & Engn Coll, Qingdao 266109, Shandong, Peoples R China;[3]Shanghai Xuhui Dist Cent Hosp, Shanghai 200031, Peoples R China

年份：2015

卷号：38

期号：1

起止页码：105

外文期刊名：BIOPROCESS AND BIOSYSTEMS ENGINEERING

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000347151300011)】；

基金：This work was financially supported by a grant from the Major State Basic Research Development Program of China (973 Program, No. 2012CB721006), National Natural Science Foundation of China (No. 21276081), the National Scientific and Technological Major Special Project (Significant Creation of New drugs, No. 2011ZX09203-001-03), and Research Fund for the Doctoral Program of Higher Education of China (No. 20110074110015).

语种：英文

外文关键词：Erythromycin; Feeding strategy; Propanol; Respiratory quotient

摘要：In this paper, glucose respiratory quotient (RQ)-feedback control was developed for erythromycin production with a recombinant strain Saccharopolyspora erythraea ZL1004. RQ was confirmed to be an ideal online parameter for regulating glucose feed rate. Through feeding glucose to control RQ at 0.85 during 45-100 h and 0.95 during 100-185 h, erythromycin titer and erythromycin A concentration were reached 11.88 and 8.82 g l(-1) in 50 l fermenter, which were increased by 8.3 and 6.1 % as compared to that with glucose pH-feedback control, respectively. When glucose RQ-feedback control was scaled up to 372-m(3) fermenter, erythromycin titer and erythromycin A concentration at 155 h were reached 9.12 and 7.12 g l(-1), respectively, which were 10.5 and 9.4 % higher than that with the original technology (glucose pH-feedback control). To the best of our knowledge, this is the first report on the successful application of glucose RQ-feedback control in erythromycin production, especially in 372-m(3) fermenter.