文献类型：期刊文献

英文题名：Repeated mutagenic effects of 60Co-γ irradiation coupled with high-throughput screening improves lipid accumulation in mutant strains of the microalgae Chlorella pyrenoidosa as a feedstock for bioenergy

作者：Wang, Weiliang[1];Wei, Tingting[1];Fan, Jianhua[1];Yi, Jun[1];Li, Yuanguang[1];Wan, Minxi[1];Wang, Jun[2];Bai, Wenmin[2]

机构：[1]East China Univ Sci & Technol, Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]JiaxingZeyuan Bioprod Ltd Co, Yatai Rd 778, Jiaxing 314006, Peoples R China

年份：2018

卷号：33

起止页码：71

外文期刊名：ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000438910600008)】；

基金：This research was funded by National Natural Science Foundation of China (Grant No. 31402319 and 31372548), Key Scientific and Technological Project of Shan Xi Province (Grant No. FT2014-01).

语种：英文

外文关键词：Chlorella pyrenoidosa; Co-60-gamma irradiation mutagenesis; Lipid; Bioenergy

摘要：In this study, the repeated mutagenic effects of Co-60-gamma irradiation combined with an optimized high-throughput screening method was applied to Chlorella pyrenoidosa to improve lipid productivity and characteristics for biodiesel. Two mutants of Chlorella pyrenoidosa, CP-232 and CP-269, were obtained. The lipid contents obtained from CP-232 and CP-269 were respectively increased by 20.8% and 20% under heterotrophic seed-photoautotrophic culture (HS-PC) model, and by 15% and 20.8% under photoautotrophic seed-photoautotrophic culture (PS-PC) model compared with the wild type strain in 1 L bubble column reactor. In addition, Co-60-gamma irradiation mutagenesis increased the proportion of saturated fatty acids in lipids accumulated in CP-232 and CP-269 compared to the wild type strain. In 3 L and 15 L bioreactor scale-up, lipid productivity was equally significantly increased in both CP-232 and CP-269 mutant strains. Our findings indicated that Co-60-gamma irradiation mutagenesis improves the accumulation of lipids with potential for industrial biodiesel production in mutant C. pyrenoidosa strains.