文献类型：期刊文献

英文题名：Enhancing methanogenic propionate degradation by resuscitation-promoting factors and pyruvate through strengthening syntrophic interactions in microbial community

作者：Fang, Bo[1,3];Liu, Yi-Fan[1,3];Li, Yu-Xuan[1,3];Zhou, Lei[1,3];Zu, Yao[1,3];Fu, Zi-Yue[1,3];Yang, Shi-Zhong[1,3];Gu, Ji-Dong[2];Mu, Bo-Zhong[1,3]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Guangdong Technion Israel Inst Technol, Environm Engn, 241 Daxue Rd, Shantou 515063, Guangdong, Peoples R China;[3]East China Univ Sci & Technol, Engn Res Ctr Microbial Enhanced Oil Recovery, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2026

卷号：256

外文期刊名：RENEWABLE ENERGY

收录：;EI(收录号：20252718707726);WOS:【SCI-EXPANDED(收录号:WOS:001524910400006)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 42173076, 42061134011 and 52074129) , the Natural Science Foundation of Shanghai (Grant No. 21ZR1417400) , Fundamental Research Funds for the Central Universities (No. JKJ01241714) and the Research Program of State Key Laboratory of Bioreactor Engineering to Y.F.L. and B.Z.M, the NSFC/RGC Joint Research Fund (No. 41161160560) to J.D.G.

语种：英文

外文关键词：Stimulants; Resuscitation promoting factors; Pyruvate; Propionate; Methane production

摘要：Propionate is one of the important intermediary products in the anaerobic digestion for biogas production, while the syntrophic propionate oxidation under methanogenic conditions is still a crucial rate-limiting step. In this study, five stimulants, including resuscitation-promoting factors RPFs (Rpf and YeaZ), chemical stimulants (pyruvate), growth promoter (fumarate), and quorum-sensing molecules (cAMP) were investigated on propionate degradation process. The results demonstrate that both RPFs and pyruvate significantly enhance propionate biodegradability (39.96-51.31 % improvement) and stimulate methanogenic degradation. Specifically, they increase the maximum methane production rate by 1.29-1.32-fold while shortening the lag phase from 7.2 to 13.1 days. Machine learning method-based community analysis and metagenomic analysis reveals significant contribution of the syntrophic propionate-oxidizing bacterium Syntrophobacter and hydrogenotrophic methanogen Methanocalculus, which were enriched after addition of RPFs and pyruvate, to methanogenic propionate degradation. Mesotoga, Thermovirga, and Lentimicrobium, which significantly contribute to methane, may participate in a co-metabolic pathway for acetate metabolism. Additionally, it shows the prevalent accumulation of parasitic microorganism, unclassified Parcubacteria, which encodes genes for acetate metabolism, corroborating the enhancement of acetate oxidation. This study suggests RPFs and pyruvate enhance propionate degradation by reviving functional microbes and strengthening syntrophic interactions, shedding light on core populations in mutualistic symbiosis during methanogenic propionate degradation.