文献类型：期刊文献

英文题名：Polydopamine-Decorated PLCL Conduit to Induce Synergetic Effect of Electrical Stimulation and Topological Morphology for Peripheral Nerve Regeneration

作者：Lu, Shunyi[1];Chen, Wen[2];Wang, Jiayi[1];Guo, Zilong[3];Xiao, Lan[4];Wei, Lingyu[2];Yu, Jieqin;Yuan, Ya[1];Chen, Weisin[1];Bian, Mengxuan[1];Huang, Lei[1];Liu, Yuanyuan[3];Zhang, Jian[1];Li, Yu-Lin[2,5];Jiang, Li-Bo[1]

机构：[1]Fudan Univ, Zhongshan Hosp, Dept Orthopaed Surg, Shanghai 200032, Peoples R China;[2]East China Univ Sci & Technol, Engn Res Ctr Biomed Mat, Sch Mat Sci & Engn, Key Lab Ultrafine Mat,State Key Lab Bioreactor Eng, Shanghai 200237, Peoples R China;[3]Shanghai Univ, Sch Mechatron Engn & Automat, Shanghai 200444, Peoples R China;[4]Queensland Univ Technol, Ctr Biomed Technol, Sch Mech Med & Proc Engn, Brisbane 4059, Australia;[5]Shanghai Univ, Wenzhou Inst, Wenzhou 325000, Peoples R China

年份：2023

卷号：7

期号：2

外文期刊名：SMALL METHODS

收录：;EI(收录号：20230113342810);WOS:【SCI-EXPANDED(收录号:WOS:000911011500001)】；

基金：S.L., W.C., J.W., and Z.G. contributed equally to this work. This work was supported by National Natural Science Foundation of China (Nos. 82272457, 81772317, and 51973060), "Technology Innovation Action Plan" of Shanghai Science and Technology Commission (21S11902700), Natural Science Foundation of Shanghai (21ZR1412300), Shanghai Talent Development Fund (2020067), Shanghai "Rising Stars of Medical Talent" Youth Development Program (Youth Medical Talents Specialist Program, [2020]087), and National Key R&D Program of China (2018YFE0201500).

语种：英文

外文关键词：exogenous electrical stimulation; grooved topography; nerve conduits; peripheral nerve regeneration; surface modifications

摘要：Due to the limited self-repairing capacity after peripheral nerve injuries (PNI), artificial nerve conduits are widely applied to facilitate neural regeneration. Exogenous electrical stimulation (ES) that is carried out by the conductive conduit regulates the biological behavior of Schwann cells (SCs). Meanwhile, a longitudinal surface structure counts to guide axonal growth to accelerate the end-to-end connection. Currently, there are no conduits equipped with both electrical conduction and axon-guiding surface structure. Herein, a biodegradable, conductive poly(l-lactide-co-caprolactone)/graphene (PLCL/GN) composite conduit is designed. The conduit with 20.96 +/- 1.26 MPa tensile strength has a micropatterned surface of 20 mu m groove fabricated by microimprint technology and self-assembled polydopamine (PDA). In vitro evaluation shows that the conduits with ES effectively stimulate the directional cell migration, adhesion, and elongation, and enhance neuronal expression of SCs. The rat sciatic nerve crush model demonstrates that the conductive micropatterned conduit with ES promotes the growth of myelin sheath, faster nerve regeneration, and 20-fold functional recovery in vivo. These discoveries prove that the PLCL(G)/PDA/GN composite conduit is a promising tool for PNI treatment by providing the functional integration of physical guidance, biomimetic biological regulation, and bioelectrical stimulation, which inspires a novel therapeutic approach for nerve regeneration in the future.