文献类型：期刊文献

英文题名：Mixed carbon source control strategy for enhancing alginate lyase production by marine Vibrio sp QY102

作者：Zhou, Jiushun[1];Cai, Menghao[1];Jiang, Tao[1];Zhou, Weiqiang[1];Shen, Wei[1];Zhou, Xiangshan[1];Zhang, Yuanxing[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2014

卷号：37

期号：3

起止页码：575

外文期刊名：BIOPROCESS AND BIOSYSTEMS ENGINEERING

收录：;EI(收录号：20141617593435);WOS:【SCI-EXPANDED(收录号:WOS:000333520600024)】；

基金：This work was financially supported by the Chinese National High Technology Research and Development Program (No. 2011AA090702, 2012AA092103, 2012AA092105). We thank Prof. Wengong Yu, Ocean University of China for supply of the strain.

语种：英文

外文关键词：Alginate induction; Alginate lyase; Vibrio sp QY102; Medium optimization; Mixed carbon source

摘要：Medium and culture conditions for alginate lyase production by marine Vibrio sp. QY102 were first optimized using statistical methods including Plackett-Burman design and central composite design. Then, fermentation in 5-L bioreactor showed that alginate acted as easily used carbohydrate for Vibrio sp. QY102, while starch extended its growth phase and stabilized pH variations. Thus, a novel strategy using mixed carbon sources was proposed that starch supported growth while enzyme synthesis was induced by pulse feedings of solid alginate. The optimized process followed that Vibrio sp. QY102 grew on starch until the end of the logarithmic growth phase, and then solid alginate was added as 1 g/L every 3 h. Meanwhile, initial pH 5.0 and natural pH during fermentation was favorable for alginate lyase production. After optimization, the highest alginate lyase production reached 52.8 U/mL, which was 329 % higher than the control. Finally, fermentation scale-up was performed in 30-L bioreactor and the maximum alginate lyase production was obtained as 46.8 U/mL.