文献类型：期刊文献

英文题名：Dual-Surfactant-Directed Mesoporous Metal-Alkyl-Thiol Frameworks for Enzyme-Au Nanoparticle-Coupled SERS Biosensing

作者：Liu, Ximeng[1];Xia, Fan[1];Feng, Dongxue[1];Yang, Jian[1];Feng, Chun[1];Gu, Jinlou[1]

机构：[1]East China Univ Sci & Technol, Sch Mat Sci & Engn, Key Lab Ultrafine Mat, Minist Educ, Shanghai, Peoples R China

年份：2026

外文期刊名：SMALL METHODS

收录：;EI(收录号：20262320835717);WOS:【SCI-EXPANDED(收录号:WOS:001782477500001)】；

基金：This work was financially supported by the National Natural Science Foundation of China (52472281, 22275054).

语种：英文

外文关键词：AuNPs; biosensing; metal-organic frameworks; SERS; template strategy

摘要：It is desirable yet challenging to introduce highly active alkyl thiols into hierarchically porous (HP) metal-organic frameworks. Herein, we developed a dual-surfactant co-templating strategy to construct Zr-based HP metal-alkyl-thiol frameworks (HPMATFs), where the HP structure facilitated the encapsulation of bulky biomacromolecules while the integrated alkyl thiols greatly enhanced their affinity toward noble metals. The high density of hydrophilic moieties at the periphery of the composite micelles effectively anchored the Zr precursors, preventing phase separation and enabling precise control over the mesopore sizes from 3 to 40 nm. The abundant alkyl thiols in the framework enabled the successful introduction of uniformly dispersed gold nanoparticles (AuNPs) within the HPMATFs matrix. Such integration provided abundant plasmonic sites and open mesospaces for the free diffusion of target analytes, forming an effective surface-enhanced Raman scattering (SERS) platform. Spatial co-confinement of oxidases and the SERS probe ensured an immediate cascade reaction between proximal enzyme-generated products and the SERS probe anchored on AuNPs, realizing specific and sensitive recognition of the substrate of the applied enzymes. By altering the loaded enzymes, such a sensory platform can be applied for the specific detection of diverse biomolecular targets, prefiguring their potential for point-of-care diagnostics.