文献类型：期刊文献

中文题名：Identification of a new thermostable and alkali-tolerantα-carbonic anhydrase from Lactobacillus delbrueckii as a biocatalyst for CO_(2) biomineralization

作者：Chun-Xiu Li[1,2];Xiao-Chen Jiang[1];Yong-Juan Qiu[1,2];Jian-He Xu[1,2]

机构：[1]Laboratory of Biocatalysis and Synthetic Biotechnology,State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai,200237,China;[2]Shanghai Collaborative Innovation Center for Biomanufacturing,130 Meilong Road,Shanghai 200237,China

年份：2015

卷号：2

期号：1

起止页码：423

中文期刊名：Bioresources and Bioprocessing

外文期刊名：生物资源与生物加工(英文)

基金：supported by the Ministry of Science and Technology(2011CB710800);the State Key Laboratory of Bioreactor Engineering,People’s Republic of China.

语种：英文

中文关键词：Lactobacillus delbrueckii;α-Carbonic anhydrase;Stability;Biomineralization

摘要：Background:Carbonic anhydrase(CA,EC 4.2.1.1),an ancient enzyme and the fastest among many enzymes,is a useful biocatalyst for carbon capture use and storage(CCUS).The use of alkaline buffers and high temperatures are favorable for biomineralization.Hence,the stability of CA under such harsh conditions is extremely important for its practical application.Methods and results:Herein,we report a new thermostable and alkaline-tolerantα-CA(designated as LdCA),with only 26%identity to bovine CA(BCA),which was identified by genome mining from Lactobacillus delbrueckii CGMCC 8137.It was overexpressed in Escherichia coli in a soluble form and purified to electrophoretic homogeneity by His-Trap affinity chromatography.The dimer protein had a subunit molecular weight of 23.8 kDa and showed extremely high stability at pH 6.0-11.0 and 30-60℃.Its activity was maintained even after incubation at 90℃ for 15 min.The half-lives of the enzyme measured at 30,40,and 50℃ were 630,370,and 177 h,respectively.At pH 9.0,10.0,and 11.0,its half-lives were 105,65,and 41 min,respectively.LdCA was applied at 50℃ to accelerate the formation of calcium carbonate in a vaterite phase.Conclusions:In summary,a new CA with high thermal and alkaline stability was identified from a general bacterium,demonstrating an effective strategy for discovering new and useful biocatalysts.