文献类型：期刊文献

英文题名：Dual mechanism β-amino acid polymers promoting cell adhesion

作者：Chen, Qi[1];Zhang, Donghui[2];Zhang, Wenjing[2];Zhang, Haodong[2];Zou, Jingcheng[2];Chen, Mingjiao[3];Li, Jin[3];Yuan, Yuan[2];Liu, Runhui[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Res Ctr Biomed Mat, Sch Mat Sci & Engn,Key Lab Ultrafine Mat,Minist E, Shanghai 200237, Peoples R China;[3]Shanghai Jiao Tong Univ, Peoples Hosp 9, Sch Med, Shanghai Key Lab Orbital Dis & Ocular Oncol,Dept, Shanghai 200011, Peoples R China

年份：2021

卷号：12

期号：1

外文期刊名：NATURE COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000684834100007)】；

基金：We thank Dr. Yibing Wang and Qize Xuan at East China University of Science and Technology for their help on surface characterization using AFM. This research was supported by the National Natural Science Foundation of China for Innovative Research Groups (No. 51621002), the National Natural Science Foundation of China (No. 21774031, 31800801), the National Key Research and Development Program of China (2016YFC1100401), the Natural Science Foundation of Shanghai (18ZR1410300), Program of Shanghai Academic/Technology Research Leader (20XD1421400), Research program of State Key Laboratory of Bioreactor Engineering, and the Fundamental Research Funds for the Central Universities (22221818014). We thank the beamline BL13W of the Shanghai Synchrotron Radiation Facility (SSRF, Shanghai, People's Republic of China) for the help with the micro-CT analysis. We also thank the Research Center of Analysis and Test of East China University of Science and Technology for the help with the characterization.

语种：英文

摘要：Cell adhesion has tremendous impact on the function of culture platforms and implants. Cell-adhesive proteins and peptides have been extensively used for decades to promote cell adhesion, however, their application suffers from their easy enzymatic degradation, difficulty in large-scale preparation and expensiveness. To develop the next-generation cell-adhesive materials, we mimic the cell adhesion functions and mechanisms of RGD and KRSR peptides and design cell-adhesive cationic-hydrophobic amphiphilic beta -amino acid polymers that are stable upon proteolysis and easily prepared in large scale at low cost. The optimal polymer strongly promotes cell adhesion, using preosteoblast cell as a model, by following dual mechanisms that are independent of sequence and chirality of the statistic copolymer. Our strategy opens avenues in designing the next-generation cell-adhesive materials and may guide future studies and applications. Cell adhesion peptides like RGD are important to biomedical applications but suffer from proteolysis as well as processing and cost issues. Here, the authors report on the development of cationic-hydrophobic amphiphilic beta -amino acid polymers which function as cell adhesion motifs but are resistant to proteolysis.