文献类型：期刊文献

英文题名：Multicoloured fluorescent indicators for live-cell and in vivo imaging of inorganic mercury dynamics

作者：Tao, Rongkun[1,3];Shi, Mei[1,3];Zou, Yejun[1,3];Cheng, Di[4];Wang, Qiaohui[1,3];Liu, Renmei[1,3];Wang, Aoxue[1,3];Zhu, Jiahuan[1,3];Deng, Lei[1,3];Hu, Hanyang[1,3];Chen, Xianjun[1,3];Du, Jiulin[2];Zhu, Weiping[4];Zhao, Yuzheng[1,3];Yang, Yi[1,2]

机构：[1]East China Univ Sci & Technol, Synthet Biol & Biotechnol Lab, State Key Lab Bioreactor Engn, Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, State Key Lab Neurosci, CAS Ctr Excellence Brain Sci, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China;[3]East China Univ Sci & Technol, Shanghai Key Lab New Drug Design, Sch Pharm, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Shanghai Key Lab Chem Biol, Sch Pharm, 130 Mei Long Rd, Shanghai 200237, Peoples R China

年份：2018

卷号：121

起止页码：26

外文期刊名：FREE RADICAL BIOLOGY AND MEDICINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000433208200004)】；

基金：We thank Robert E. Campbell for the R-GECO vector; Peng Chen and Chuan He for the cDNA of MerR. This research was supported by National Key Research and Development Program of China (2017YFC0906900, 2017YFA050400), NSFC (31722033, 91649123, 31671484, 31225008, and 31470833), the Shanghai Science and Technology Commission (14XD1401400, 16430723100, and 15YF1402600), Young Elite Scientists Sponsorship Program by Cast (YESS20150027), Shanghai Young Top-notch Talent (to Y. Zhao), the State Key Laboratory of Bioreactor Engineering (to Y.Y.), and the Fundamental Research Funds for the Central Universities (to Y.Y. and Y. Zhao).

语种：英文

外文关键词：Inorganic mercury; Multicoloured fluorescent indicators; Live-cell imaging; In vivo imaging; Oxidative damage

摘要：Engineered fluorescent indicators for visualizing mercury ion (Hg2+) are powerful tools to illustrate the intracellular distribution and serious toxicity of the ion. However, the sensitive and specific detection of Hg2+ in living cells and in vivo is challenging. This paper reported the development of fluorescent indicators for Hg2+ in green or red color by inserting a circularly permuted fluorescent protein into a highly mercury-specific repressor. These sensors provided a rapid, sensitive, specific, and real-time read-out of Hg2+ dynamics in solutions, bacteria, subcellular organelles of mammalian cells, and zebrafish, thereby providing a useful new method for Hg2+ detection and bioimaging. In conjunction with the hydrogen peroxide sensor HyPer, we found mercury uptake would trigger subcellular oxidative events at the single-cell level, and provided visual evidence of the causality of mercury and oxidative damage. These sensors would paint the landscape of mercury toxicity to cell functions.