文献类型：期刊文献

英文题名：Single-step fabrication of catechol-e-poly-L-lysine antimicrobial paint that prevents superbug infection and promotes osteoconductivity of titanium implants

作者：Xu, Miao[1,2];Song, Qing[1];Gao, Lingling[1,2];Liu, Huan[3];Feng, Wei[1,2];Huo, Jingjing[1];Jin, Haoyu[1];Huang, Lei[4];Chai, Jin[1];Pei, Yangyang[1];Qu, Xue[3];Li, Peng[1,2];Huang, Wei[1,2]

机构：[1]Northwestern Polytech Univ, Shaanxi Inst Biomed Mat & Engn SIBME, Shaanxi Inst Flexible Elect SIFE, Frontiers Sci Ctr Flexible Elect FSCFE, 127 West Youyi Rd, Xian 710072, Peoples R China;[2]Nanjing Tech Univ NanjingTech, Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Inst Adv Mat IAM, Key Lab Flexible Elect KLOFE, Nanjing 211816, Peoples R China;[3]East China Univ Sci & Technol, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[4]Guizhou Med Univ, State Key Laboratroy Funct & Applicat Med Plants, Guiyang 550014, Peoples R China

年份：2020

卷号：396

外文期刊名：CHEMICAL ENGINEERING JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000551969200068)】；

基金：This work was financially supported by the National Key R&D Program of China (2018YFC1105402 and 2017YFA0207202); the National Natural Science Foundation of China (21875189 and 21706222); Key R&D Program of Jiangsu Province, China (BE2017740); Innovative Talents Promotion Project of Shaanxi Province, China (2019KJXX-064); and the Fundamental Research Funds for the Central Universities, China.

语种：英文

外文关键词：Surface modification; Mussel-inspired; Antimicrobial peptides; Osteoconduction; Bio-inspired mineralization

摘要：Prevention of infection and promotion of osteoconduction are the key factors to achieve long-term success in orthopaedic implants. In this work, an effective antimicrobial and osteoconductive coating is facilely prepared by the cross-linking of epsilon-poly-L-lysine (EPL) and catechol (abbreviated as EPLC) via a one-step mussel-inspired chemistry. A series of EPLC paints were prepared by catechol (15 mM) and varying concentrations of EPL (30, 40, and 50 mM) and named as EPLC1, EPLC2, and EPLC3. The coatings were easy to paint on implants. EPLC3 with the highest EPL concentration exhibited the most potent antimicrobial activity (greater than 99.99%) against both Gram-negative bacteria Escherichia coli (E. coli) and Gram-positive "superbug" methicillin-resistant Staphylococcus aureus (MRSA), and inhibited the biofilm formation of MRSA up to one week. Furthermore, the EPLC3 painted Ti implant was capable of reducing more than 3-log bacterial burden in a rodent subcutaneous infection model infected by MRSA. More interestingly, EPLC3 coating increased the osteoconductivity of Ti surfaces by promoting mouse pre-osteoblast MC3T3-E1 adhesion and proliferation. And the EPLC3 painted Ti surfaces could induce hydroxyapatite (HAp) formation after incubation in simulating human body fluids (SBF) within two weeks. Overall, the mussel-inspired EPLC paint can be easily applied to orthopaedic implants, and uendowed Ti with antimicrobial activity and promoted its osteoconductivity, which will benefit the long-term success of Ti implants.