文献类型：期刊文献

英文题名：Immobilization of cellulase onto a recyclable thermo-responsive polymer as bioconjugate

作者：Ding, Zhoyang[1];Zheng, Xuexuan[1];Li, Sipeng[1];Cao, Xuejun[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Dept Bioengn, Shanghai 200237, Peoples R China

年份：2016

卷号：128

起止页码：39

外文期刊名：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

收录：;EI(收录号：20161302157338);WOS:【SCI-EXPANDED(收录号:WOS:000375008100005)】；

基金：This research was supported by the Key Laboratory for Ultrafine Materials of The Ministry of Education, East China University of Science and Technology (ECUST).

语种：英文

外文关键词：Thermo-responsive polymer; Recyclable; Immobilization; Cellulase

摘要：Here we report a recyclable thermo-responsive polymer-cellulase bioconjugate, a recoverable, thermo-responsive polymer (P-NMN) which was synthesized through the polymerization of N-isopropylmethacrylamide (NIPMA) with methyl acrylate and N-(Hydroxymethyl) acrylamide was used for connection with cellulase to form bioconjugate. The polymer exhibited a thermo-responsive lower critical solution temperature (LCST). The aminooxy group of P-NMN provided a handle through which the LCST was adjusted through small-molecule quenching. This allowed the polymer to have a LCST of 51.6 degrees C and recovery of 98.5%. Cellulase was covalently combined to P-NMN by 1-ethyl-3-(3-dimethyllaminopropyl) carbodiimide as bioconjugate (P-NMN-C). The surface morphologies of P-NMN and PNMN-C were obtained by scanning electron microscope (SEM). Under optimized conditions, the highest activity yield of polymer-cellulase bioconjugate construction was 83.2%. Maximum activity of the polymer-cellulase bioconjugate was achieved at 50.0 degrees C (pH 5.0), while free cellulase exhibited maximum activity at 55.0 degrees C (pH 5.0). The polymer-cellulase bioconjugate retained 85.2% of its initial activity after repeated five cycles of hydrolysis reaction. P-NMN-C could be dissolved and used efficiently during 50.0 degrees C, and could be collected as precipitate higher than LCST. P-NMN is a promising carrier for immobilizing cellulase. (C) 2016 Elsevier B.V. All rights reserved.