文献类型：期刊文献

英文题名：Metabolically engineering of Yarrowia lipolytica for the biosynthesis of naringenin from a mixture of glucose and xylose

作者：Wei, Wenping[1];Zhang, Ping[1];Shang, Yanzhe[1];Zhou, Ying[1];Ye, Bang-Ce[1,2]

机构：[1]East China Univ Sci & Technol, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Zhejiang Univ Technol, Coll Pharmaceut Sci, Collaborat Innovat Ctr Yangtze River Delta Reg Gr, Inst Engn Biol & Hlth, Hangzhou 310014, Zhejiang, Peoples R China

年份：2020

卷号：314

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20202708889469);WOS:【SCI-EXPANDED(收录号:WOS:000559022800002)】；

基金：This work was supported by the National Key Research and Development Project [2018YFA0900404] and National Natural Science Foundation of China [31730004].

语种：英文

外文关键词：Naringenin; Natural product biosynthesis; Xylose-inducible; Xylose utilization; Yarrowia lipolytica

摘要：Xylose-inducible modules simultaneously expressing xylose utilization and naringenin biosynthesis pathways were developed in Yarrowia lipolytica to produce naringenin from a mixture of glucose and xylose. The naringenin synthetic pathway was constructed using a constitutive expression to yield 239.1 +/- 5.1 mg/L naringenin. Furthermore, the introduction of an inducible pathway realized the dual function of xylose as a substrate and synthetic inducer, which coupled the xylose utilization with naringenin biosynthesis and increased production. Interestingly, the simultaneous enhancement of xylose reductase and xylose transporter expression along with that of xylitol dehydrogenase and xylulokinase can further improve the xylose utilization ability of Y. lipolytica. As expected, xylose-inducible synthesis of naringenin could achieved a titer of 715.3 +/- 12.8 mg/L through the shake-flask cultivation level. Therefore, xylose-induced activation of both the xylose utilization and product biosynthesis pathway is considered to be an effective strategy for the biosynthesis of xylose-derived chemicals in yeast.