文献类型：期刊文献

中文题名：CA-HA/DTAB复合物对CA-HA在磷脂膜表面渗透作用的影响

英文题名：Effects of the Formation of CA-HA/DTAB Complexes on the Permeability of CA-HA on the Surface of Phospholipid Membrane

作者：冯海飞[1];井艺霏[1];王小永[1]

机构：[1]华东理工大学化学与分子工程学院,上海200237

年份：2025

卷号：51

期号：4

起止页码：446

中文期刊名：华东理工大学学报(自然科学版)

外文期刊名：Journal of East China University of Science and Technology

收录：;北大核心:【北大核心2023】；

基金：国家自然科学基金(22273022)。

语种：中文

中文关键词：透明质酸;疏水改性;表面活性剂;静电吸引;累积渗透量

外文关键词：hyaluronic acid;hydrophobic modification;surfactant;electrostatic attraction;cumulative permeation amount

摘要：随着十二烷基三甲基溴化铵(DTAB)浓度的增加,肉桂酸疏水改性透明质酸(CA-HA)与DTAB通过静电和疏水作用生成4种CA-HA/DTAB复合物(分别表示为CA-HA/DTAB(Ⅰ)、CA-HA/DTAB(Ⅱ)、CA-HA/DTAB(Ⅲ)、CA-HA/DTAB(Ⅳ))。使用透射电镜、动态光散射、Zeta电位和紫外吸收光谱方法,研究了不同结构CA-HA/DTAB复合物的生成及转变机理。在CA-HA/DTAB(Ⅰ)中,CA-HA在磷脂膜表面的渗透速率和平衡累积渗透量分别为游离CA-HA对应值的2.0倍和1.5倍;然而,在CA-HA/DTAB(Ⅱ)、CA-HA/DTAB(Ⅲ)和CA-HA/DTAB(Ⅳ)中,CA-HA在磷脂膜表面基本没有渗透作用。讨论了4种CA-HA/DTAB复合物对CA-HA在磷脂膜表面产生不同渗透作用的原因。
With the increase of the concentration of dodecyl trimethyl ammonium bromide(DTAB),hyaluronic acid(CA-HA)hydrophobically modified with cinnamic acid forms four kinds of CA-HA/DTAB complexes(Ⅰ,Ⅱ,Ⅲ,Ⅳ)through electrostatic and hydrophobic interactions.The formation and transformation mechanisms of CA-HA/DTAB complexes with different structures have been studied by means of transmission electron microscopy,dynamic light scattering,Zeta potential measurement and UV absorption spectroscopy.CA-HA/DTAB(Ⅰ)is formed by the electrostatic attraction between the positively charged headgroup of DTAB and the negatively charged carboxyl groups in CA-HA.The hydrophobic aggregation of the DTAB alkyl chain causes the molecular chain of CA-HA to cross-link,leading to the transformation of CA-HA/DTAB(Ⅰ)into CA-HA/DTAB(Ⅱ).In CA-HA/DTAB(Ⅲ),DTAB forms micelles on the molecular chain of CA-HA.The electrostatic repulsion between DTAB micelles disintegrates CA-HA molecular chains to form CA-HA/DTAB(Ⅳ).Through a permeation experiment on the surface of phospholipid membrane,it is found that the permeation rate and equilibrium cumulative permeation amount of CA-HA in CA-HA/DTAB(Ⅰ)were 2.0 times and 1.5 times those of pure CA-HA,respectively,and CA-HA in CA-HA/DTAB(Ⅱ),CA-HA/DTAB(Ⅲ),and CA-HA/DTAB(IV)have almost no permeation ability.The different permeability of CA-HA/DTAB(Ⅰ),CA-HA/DTAB(Ⅱ),CA-HA/DTAB(Ⅲ),and CA-HA/DTAB(Ⅳ)is rationalized.Because of a small particle size and high flexibility,CA-HA/DTAB(Ⅰ)renders CA-HA more permeable,and its equilibrium cumulative permeation amount on the phospholipid membrane surface is higher than that of pure CA-HA.Due to the large aggregation structures of CA-HA/DTAB(Ⅱ)and CA-HA/DTAB(Ⅲ),CA-HA is not able to penetrate the phospholipid membrane.CA-HA/DTAB(Ⅳ)can clog the phospholipid membrane pore by forming a film on the membrane surface,which can thoroughly inhibit the permeation of CA-HA through the phospholipid membrane surface.