文献类型：期刊文献

英文题名：Engineering organelle-specific activatable molecules for ultra-fast and reliable in situ mapping of subcellular nitric oxide

作者：Sun, Lixin[1,2];Wang, Xinyu[1,2];Chen, Rui[1,2];Dong, Xuemei[1,2];Sun, Jie[1,2];Dong, Chengjun[1,2];Xie, Haijiao[4];Gu, Xianfeng[3];Zhao, Chunchang[1,2]

机构：[1]East China Univ Sci & Technol, Inst Fine Chem, Frontiers Sci Ctr Materiobiol & Dynam Chem, Inst Fine Chem,Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Inst Fine Chem, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,Feringa Nobel Prize Scientist, Shanghai 200237, Peoples R China;[3]Fudan Univ, Sch Pharm, Dept Med Chem, Shanghai 201203, Peoples R China;[4]Hangzhou Yanqu Informat Technol Co Ltd, Y2,2nd Floor,Bldg 2,Xixi Legu Creat Pioneering Pk,, Hangzhou 310003, Zhejiang, Peoples R China

年份：2024

卷号：12

期号：9

起止页码：2304

外文期刊名：JOURNAL OF MATERIALS CHEMISTRY B

收录：;EI(收录号：20240815567935);WOS:【SCI-EXPANDED(收录号:WOS:001161635300001)】；

基金：We gratefully acknowledge financial support by the National Natural Science Foundation of China (22077030, 22271092, 21977018, and 82173657). We thank the Research Center of Analysis and Test of East China University of Science and Technology for the help on the characterization.

语种：英文

外文关键词：Free radical reactions - Free radicals - Mapping - Probes

摘要：Nitric oxide (NO), a ubiquitous gaseous transmitter in living systems, is closely associated with physiopathological processes in the endoplasmic reticulum and lysosomes. This free radical gas is very widely but very heterogeneously distributed in the biological microenvironment, which poses a great challenge to specifically detect its localized levels in certain subcellular regions. In this study, we proposed six subcellular targeting probes by rational molecular engineering and selected two probes with optimal performance for the precise spatiotemporal identification of endoplasmic reticulum (ER) and lysosomal NO fluctuations. The probes could rapidly undergo a N-nitrosation reaction with NO at a riveted subcellular location, blocking the initial photoinduced electron transfer (PET) process and generating bright fluorescence for precise mapping of NO in the ER and lysosomes. The screened probes have ultra-sensitive reactivity and ultra-low detection limits for NO, realizing the precise depiction of exogenous and endogenous NO in the corresponding subcellular area. Fluctuations in the subcellular levels of NO during inflammation were also successfully mapped by the probes. Our work will contribute to the accurate study of the physiological and pathological consequences of subcellular NO in various biological events.