文献类型：期刊文献

英文题名：Rapid analysis of asarone and asaryladehyde in fructus carotae by LC

作者：Lu, Yan-Hua[1]; Wang, Han-Jing[1]; Wang, Zheng-Tao[2]; Wei, Dong-Zhi[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; [2] Institute of Chinese Materia Medica, Shanghai University, TCM, Shanghai 201203, China

年份：2009

卷号：69

期号：5-6

起止页码：549

外文期刊名：Chromatographia

收录：EI(收录号：20242616433957)

语种：英文

外文关键词：Column chromatography - Liquid chromatography - Medicine - Plant extracts

摘要：Fructus carotae (Nan Heshi in Chinese) has been used as an insecticidal, pain-relief, antiphlogistic drug and vermifugum in traditional Chinese herbal medicines for a long time. To evaluate the quality of Fructus carotae, a sensitive, simple and accurate liquid chromatographic (LC) method was developed for the determination of two main bioactive compounds, namely asarone and asaryladehyde. The chromatographic separation was performed on an Agilent SB-C18 column (250 × 4.6 mm, 5.0 μm) with a gradient elution program using a mixture of acetonitrile and redistilled water (v/v) as mobile phase within 30 min at 237 nm wavelength. The two compounds showed good linear relation (R 2 > 0.999) in the range of 0.082-74.3 μg mL -1 for asaryladehyde and 0.703-87.639 μg mL-1 for asarone. The recovery of the method was in the range of 95.9%-103.7%. Nine Fructus carotae samples from different regions of China were analyzed to establish the effectiveness of the method. These studies could be useful for the quality control of Fructus carotae and its related traditional Chinese medicinal preparations. ? 2009 Vieweg+Teubner | GWV Fachverlage GmbH.