文献类型：期刊文献

英文题名：Enhanced lipid productivity of Chlorella pyrenoidosa through the culture strategy of semi-continuous cultivation with nitrogen limitation and pH control by CO2

作者：Han, Feifei[1];Huang, Jianke[1];Li, Yuanguang[1];Wang, Weiliang[1];Wan, Minxi[1];Shen, Guomin[1];Wang, Jun[2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Jiaxing Zeyuan Bioprod Ltd Co, Jiaxing 314006, Peoples R China

年份：2013

卷号：136

起止页码：418

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20131716231162);WOS:【SCI-EXPANDED(收录号:WOS:000319309400057)】；

基金：This research was funded by National Basic Research Program of China (973 Program: 2011CB200904 and 2011CB200903), National High Technology Research and Development Program of China (863 Program: 2012AA050101, 2013AA065804), National Key Technologies R&D Program (2011BAD14B02 and 2011BAD23B04) and Grant No.GHME2011SW03.

语种：英文

外文关键词：Chlorella; pH control; Nitrogen limitation; Semi-continuous culture; Lipid productivity

摘要：Microalgae cultivation with high lipid productivity has received much attention in recent years owing to the economic potential of biofuels production and CO2 emission reduction. Previous studies had reported that the ways of pH-regulation, nutrition-limitation and semi-continuous culture mode can either increase the cells growth rate or promote lipid accumulation. In this study, the novel culture strategy of integrating both nutrition limitation and pH-regulation by CO2 in a semi-continuous cultivation was investigated for enhancing the lipid productivity. In the batch culture, the best growth performance was achieved by controlling pH at 7; lipid contents of the cells can be increased under the nitrogen-limitation conditions. The maximum lipid productivity of 115 mg L-1 d(-1) was achieved in the novel culture strategy of semi-continuous cultivation with nitrogen-limitation and pH-regulation by CO2, which was 3.64-fold higher than that in the batch culture without pH control and nitrogen limitation. (C) 2013 Elsevier Ltd. All rights reserved.