文献类型：期刊文献

英文题名：Immobilization of superoxide dismutase on Pt-Pd/MWCNTs hybrid modified electrode surface for superoxide anion detection

作者：Zhu, Xiang[1];Niu, Xiangheng[1];Zhao, Hongli[1];Tang, Jie[1];Lan, Minbo[1,2]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2015

卷号：67

起止页码：79

外文期刊名：BIOSENSORS & BIOELECTRONICS

收录：;EI(收录号：20150600505487);WOS:【SCI-EXPANDED(收录号:WOS:000350076900013)】；

基金：This research was financially supported by National Natural Science Foundation of China (NSFC, No. 21305044), the Fundamental Research Funds for the Central Universities (No. 222201314026) and Science and Technology Commission of Shanghai Municipality (STCSM, No. 13510710900).

语种：英文

外文关键词：Pt-Pd/MWCNTs hybrid; Superoxide dismutase; Enzyme-based electrochemcial sensor; Superoxide anion detection; High sensitivity and stability

摘要：Monitoring of reactive oxygen species like superoxide anion (O-2(center dot-)) turns to be of increasing significance considering their potential damages to organism. In the present work, we fabricated a novel O-2(center dot-)-electrochemical sensor through immobilizing superoxide dismutase (SOD) onto a Pt-Pd/MVVCNTs hybrid modified electrode surface. The Pt-Pd/MVVCNTs hybrid was synthesized via a facile one-step alcoholreduction process, and well characterized by transmission electron microscopy, X-ray photoelectron spectroscopy and X-ray diffraction. The immobilization of SOD was accomplished using a simple dropcasting method, and the performance of the assembled enzyme-based sensor for O-2(center dot-) detection was systematically investigated by several electrochemcial techniques. Thanks to the specific biocatalysis of SOD towards O-2(center dot-) and the Pt-Pd/MWCNTs - promoted fast electron transfer at the fabricated interface, the developed biosensor exhibits a fast, selective and linear amperometric response upon O-2(center dot-) in the concentration scope of 40-1550 mu M (R-2=0.9941), with a sensitivity of 0.601 mA cm(-2) mM(-1) and a detection limit of 0.71 mu M (S/N=3). In addition, the favorable biocompatibility of this electrode interface endows the prepared biosensor with excellent long-term stability (a sensitivity loss of only 3% over a period of 30 days). It is promising that the proposed sensor will be utilized as an effective tool to quantitatively monitor the dynamic changes of O-2(center dot-) in biological systems. (C) 2014 Elsevier B.V. All rights reserved.