文献类型：期刊文献

英文题名：Role of jasmonic acid in alteration of ginsenoside heterogeneity in elicited cell cultures of Panax notoginseng

作者：Hu, Feng-Xian[2];Zhong, Jian-Jiang[1,2]

机构：[1]Shanghai Jiao Tong Univ, Coll Life Sci & Biotechnol, Key Lab Microbial Metab, Minist Educ, Shanghai 200240, Peoples R China;[2]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2007

卷号：104

期号：6

起止页码：513

外文期刊名：JOURNAL OF BIOSCIENCE AND BIOENGINEERING

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000253209800013)】；

语种：英文

外文关键词：Panax notoginseng; enzyme activity; ginsenoside heterogeneity; secondary metabolite; synthetic jasmonate derivative; plant cell culture

摘要：The efficient manipulation of ginsenoside heterogeneity of Panax notoginseng cells using a recently synthesized elicitor, 2-hydroxyethyl jasmonate (HEJ, at 200 mu M), has been reported. In this work, the activities of two enzymes related to ginsenoside heterogeneity (distribution), proto-panaxdiol 6-hydroxylase (P6H) and UDPG-ginsenoside Rd glucosyltransferase (UGRdGT), were examined in cell cultures of P notoginseng elicited by HEJ. P6H and UGRdGT activities were increased by HEJ with corresponding changes in Rb/Rg ratio and Rb1/Rd ratio. Endogenous jasmonic acid (JA) seemed to mediate the induction of UGRdGT activation, but was not involved in P6H activation. The results suggest that JA, as a signal transducer, may play an important role in the alteration of ginsenoside heterogeneity in elicited P. notoginseng cells.