文献类型：期刊文献

英文题名：Fluorescent probes for simultaneous detection of lysosomal biothiol and proton: Effects of proton receptor and spacer length on the selectivity

作者：Wang, Xiaoxiao[1];Yu, Zhihao[1];Zhang, Guangzhu[1];Zhou, Langping[1];Chen, Yixiao[1];Zhang, Weibing[1];Qian, Junhong[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China

年份：2026

卷号：349

外文期刊名：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

收录：;EI(收录号：20255119736341);WOS:【SCI-EXPANDED(收录号:WOS:001642281100001)】；

基金：This work was financially supported by National Key Research and Development Program of China (2022YFD1700400) . The authors appreciate Prof. Tanyu Cheng from Shanghai Normal University for assisting with the synthesis of NLA and NLC.

语种：英文

外文关键词：Fluorescent probe; Biothiol; Linker length; Proton receptor; Naphthalimide

摘要：Four fluorescent probes based on "proton receptor-spacer1-fluorophore-spacer2-thiol receptor" model were synthesized for simultaneous detection of proton and thiols. The probes were constructed with naphthalimide as the fluorophore, maleimide and N-(2-aminoethyl)morpholine (NLA, NLB)/4-(dimethylamino)benzyl (NLC, NLD) as the receptors for thiol and proton, respectively. The linker length between the thiol receptor and the fluorophore (spacer2) is essential to the selectivity of the probe toward thiols: the probes with a shorter spacer2 (NLA, NLC) exhibited high selectivity to Cys and could image exogenous Cys in L929 cells; while those with a longer linker (NLB, NLD) responded to all three thiols (Cys, GSH and Hcy) and could image lysosomal thiols in living cells. The electron effect of proton receptor affected its electron transfer efficiency to the fluorophore (a-PET), which in turn influenced the response of the probe toward H+. The probes bearing 4-(dimethylamino)benzyl (NLC and NLD) implemented "AND" logical function with thiols and H+ as the inputs, whereas those containing morpholine (NLA and NLB) could be applied as highly selective probes for thiols.