文献类型：专利

英文题名：Isolating and purifying insect transglutaminase, by isolating insect glutamine transaminase by precipitation using ammonium sulfate and obtaining target protein by chromatography on glucose gel column and ion-exchange column

作者：HUANG Q;ZHANG L;YUN X;RAO W;XIAO C;YANG C

机构：[1]UNIV EAST CHINA SCI & TECHNOLOGY

申请号：CN106011099-A

申请日：2016-06-23

公开日：2016-10-12

语种：英文

收录：DERWENT

摘要：NOVELTY - Method for isolating and purifying insect transglutaminase, involves preparing insect transglutaminase, isolating an insect glutamine transaminase by precipitation using ammonium sulfate to obtain preliminary target protein, and obtaining target protein by chromatography on glucose gel column and the ion-exchange column in turn, or obtaining target protein through ion exchange column and the glucose gel column chromatography sequentially in primary target protein, where the insects are Lepidoptera, Orthoptera, Coleoptera, Diptera, Homoptera or Diptera pests. USE - The method is useful for isolating and purifying insect transglutaminase (claimed). DETAILED DESCRIPTION - Method for isolating and purifying insect transglutaminase, involves preparing insect transglutaminase, isolating an insect glutamine transaminase by precipitation using ammonium sulfate to obtain preliminary target protein, and obtaining the target protein by chromatography on the glucose gel column and the ion-exchange column in turn, or obtaining the target protein through ion exchange column and the glucose gel column chromatography sequentially in the primary target protein, where the insects are Lepidoptera, Orthoptera, Coleoptera, Diptera, Homoptera or Diptera pests, and the separation range of the glucose gel column is 3000-150000.