文献类型：期刊文献

英文题名：A Mitochondrial-targeted curcumin-based fluorescent probe for real-time tracking endogenous hydrogen peroxide in living cells

作者：Zhu, Mingming[1];Wang, Ting[2];Zhang, Yutao[2];Nong, Jiahuan[2];Guo, Zhiqian[2]

机构：[1]Shanghai Jiao Tong Univ, Renji Hosp, Shanghai Inst Digest Dis, Inflammatory Bowel Dis Res Ctr,Div Gastroenterol &, 145 Middle Shandong Rd, Shanghai 200001, Peoples R China;[2]East China Univ Sci & Technol, Inst Fine Chem, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,State Key Lab Bioreactor Engn,, Shanghai 200237, Peoples R China

年份：2023

卷号：391

外文期刊名：SENSORS AND ACTUATORS B-CHEMICAL

收录：;EI(收录号：20232214170220);WOS:【SCI-EXPANDED(收录号:WOS:001053339700001)】；

基金：Acknowledgements This work was supported by NSFC/China (22225805 and 32121005) , Shanghai Pujiang Program (Grant 21PJD038) , Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell Metabolism (Shanghai Municipal Education Commission, grant 2021 Sci & Tech 03-28) .

语种：英文

外文关键词：Fluorescent probe; Dual -channel; Near -infrared; Hydrogen peroxide; Mitochondrialtargeting

摘要：To explore high-performance fluorescent probes that endow tunability and capability for in vivo analysis is an enduring goal of visualizing and manipulating biological events. Hydrogen peroxide H2O2, as a central redox signaling molecule, is the tough hurdle to real-time in situ tracking dynamic subcellular H2O2. Herein, we present a curcuminoid difluoroboron based fluorescent probe DFB-1 which contains the triphenylphosphine (TPP) moiety as mitochondrial targeting unit and aryl boronates as the specific uncaging group. Notably, this activatable probe DFB-1 bestows several striking characteristics: (i) dual-channel fluorescence response with a large shift; (ii) active mitochondria-targeting; (iii) light-up NIR fluorescence with high signal-to-background ratio. The probe DFB-1 is successfully employed in sensing endogenous H2O2 with a limit of detection 0.025 & mu;M, and effectively differentiate H2O2 fluctuation between hepatocellular carcinoma and colorectal cancer cells. This dual-channel NIR probe DFB-1 realizes new advances in deep insight into the function of mitochondrial endogenous H2O2.