文献类型：期刊文献

英文题名：Development of a Method to Extract and Purify Target Compounds from Medicinal Plants in a Single Step: Online Hyphenation of Expanded Bed Adsorption Chromatography and Countercurrent Chromatography

作者：Li, Yang[1];Wang, Nan[1];Zhang, Min[2,3];Ito, Yoichiro[3];Zhang, Hongyang[1];Wang, Yuerong[1];Guo, Xin[1];Hu, Ping[1]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, Modern Engn Ctr Tradit Chinese Med, Shanghai 200237, Peoples R China;[3]NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA

年份：2014

卷号：86

期号：7

起止页码：3373

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20141517562244);WOS:【SCI-EXPANDED(收录号:WOS:000333776600019)】；

基金：This research was financially supported by the National Natural Science Foundation of China (No. 81273481 and 21006023), the Fundamental Research Funds from the Central Universities, and National Science and Technology Major Project for Significant New Drugs Development (No. 2013ZX09507005).

语种：英文

外文关键词：Liquid chromatography - Drug products - Plant extracts

摘要：Pure compounds extracted and purified from natural sources are crucial to lead discovery and drug screening. This study presents a novel two-dimensional hyphenation of expanded bed adsorption chromatography (EBAC) and high-speed countercurrent chromatography (HSCCC) for extraction and purification of target compounds from medicinal plants in a single step. The EBAC and HSCCC were hyphenated via a six-port injection valve as an interface. Fractionation of ingredients of Salvia miltiorrhiza and Rhizoma coptidis was performed on the hyphenated system to verify its efficacy. Two compounds were harvested from Salvia miltiorrhiza, one was 52.9 mg of salvianolic acid B with an over 95% purity and the other was 2.1 mg of rosmarinic acid with a 74% purity. Another two components were purified from Rhizoma coptidis, one was 4.6 mg of coptisine with a 98% purity and one was 4.1 mg of berberine with a 82% purity. The processing time was nearly 50% that of the multistep method. The results indicate that the present method is a rapid and green way to harvest targets from medicinal plants in a single step.