文献类型：期刊文献

英文题名：A potential-resolved electrochemiluminescent aptasensor for simultaneously detecting MUC1 and HER2 on breast cancer exosomes

作者：Jiang, Dong[1];Li, Rui[2];Wang, Qingjiang[2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]East China Normal Univ, Sch Chem & Mol Engn, Shanghai 200241, Peoples R China

年份：2026

卷号：18

期号：10

起止页码：2122

外文期刊名：ANALYTICAL METHODS

收录：;EI(收录号：20260920164404);WOS:【SCI-EXPANDED(收录号:WOS:001701585100001)】；

基金：This work was financially supported by the National Natural Science Foundation of China (Grant No. 22074042).

语种：英文

外文关键词：Cancer cells - Diseases - Electrodes - Emitter coupled logic circuits - Energy transfer - Indium compounds - Lung cancer - Nanocomposites - Nanoprobes - Proteins - SiO2 nanoparticles - Tin oxides - TiO2 nanoparticles

摘要：Breast cancer is one of the most serious threats to women's health. The simultaneous detection of tumor markers is crucial for improving the survival rate of patients. Herein, a potential-resolved electrochemiluminescent (ECL) aptasensor has been developed for the simultaneous detection of MUC1 and HER2 proteins on the surface of breast cancer cell-derived exosomes. Exosomes were specifically captured via CD63 aptamers immobilized on an indium tin oxide (ITO) electrode. Ru(bpy)32+@SiO2 nanoparticles (Ru@SiO2) and luminol@Au nanoparticles (lum@Au) labeled with the corresponding aptamers of the MUC1 and HER2 proteins, respectively, were employed as ECL nanoprobes to specifically recognize these two proteins expressed on exosomes, and were anchored on two independent regions of an ITO electrode patterned by polydimethylsiloxane (PDMS). This physical isolation eliminated ECL resonance energy transfer (ECL-RET) between the nanoprobes. The ECL behaviors of the two nanoprobes at different potentials were recorded under cyclic voltammetry scanning, achieving the simultaneous detection of both proteins. Under the optimized conditions, the ECL aptasensor exhibited favorable linear response relationships for MUC1 and HER2 on four types of exosomes, thus enabling the quantitative analysis and the reliable discrimination of these distinct exosomal subtypes. Endowed with excellent specificity, stability and reproducibility, this aptasensor was further applied to the detection of serum samples, demonstrating potential for clinical application.