文献类型：期刊文献

英文题名：Characterization of essential elements for improved episomal expressions in Kluyveromyces marxianus

作者：Wu, Pingping[1,2];Zhou, Jungang[1,2];Yu, Yao[1,2,4];Lu, Hong[1,2,3,4]

机构：[1]Fudan Univ, Sch Life Sci, State Key Lab Genet Engn, Shanghai 200438, Peoples R China;[2]Shanghai Engn Res Ctr Ind Microorganisms, Shanghai, Peoples R China;[3]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg SCICB, Shanghai, Peoples R China;[4]Natl Technol Innovat Ctr Synthet Biol, Tianjin, Peoples R China

年份：2022

卷号：17

期号：4

外文期刊名：BIOTECHNOLOGY JOURNAL

收录：;EI(收录号：20220611603030);WOS:【SCI-EXPANDED(收录号:WOS:000750486900001)】；

基金：National Key Research and Development Program of China, Grant/Award Number: 2021YFC2100204; Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project, Grant/Award Number: TSBICIP-006; National Natural Science Foundation of China, Grant/Award Number: 31970549; Science and Technology Research Program of Shanghai, Grant/Award Number: 19DZ2282100

语种：英文

外文关键词：episomal expression; Kluyveromyces marxianus; pKD1; promoter; terminator

摘要：Essential components including partition element, copy number control element, promoter, and terminator in multicopy plasmids applicable for K. marxianus are not extensively characterized. The pKD1 is an endogenous multicopy plasmid identified in K. lactis, and the pKD1-based plasmid is the only multicopy plasmid successfully applied in K. marxianus. The circular plasmid pKD1 contains three major open reading frames, namely A, B, and C. Here, we showed that the A gene was responsible for maintaining the high-copy number of pKD1-based plasmid in K. marxianus. Deletion of the B or C gene impaired the stable propagation of pKD1-based plasmid in K. marxianus, and this defect could not be rescued by the trans expression of B and C genes. In a quantitative analysis of a series of promoters and terminators, AFT1 promoter from Pichia pastoris, OM45 promoter and INU1 terminator from K. marxianus, and a set of synthetic terminators, supported high-level expressions. A combination of AFT1 or OM45 promoter with INU1 terminator in a pKD1-based plasmid achieved high-level expressions of four different heterologous proteins. Our study provides valuable elements for high-level episomal expressions in K. marxianus.