文献类型：期刊文献

英文题名：Fabrication and in vitro evaluation of a packed-bed bioreactor based on galactosylated poly(ethylene terephthalate) microfibrous scaffolds

作者：Liu, Wei[1];Zhang, Mi[1];Xiao, Yan[2];Ye, Zhaoyang[1];Zhou, Yan[1];Lang, Meidong[2];Tan, Wen-Song[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Mat Sci & Engn, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China

年份：2020

卷号：160

外文期刊名：BIOCHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20202108694309);WOS:【SCI-EXPANDED(收录号:WOS:000542595600001)】；

基金：This work was financially supported by the National Key Research and Development Program of China (Grant No. 2018YFC1105800), the National Natural Science Foundation of China (Grant No. 81671841), the Fundamental Research Funds for the Central Universities (Grant No. 22221818014), and the Basic Research Project of Shanghai Science and Technology Commission (Grant No. 16JC1400203).

语种：英文

外文关键词：Packed-bed bioreactor; Perfusion culture; Galactosylated microfibrous scaffolds; Hepatocyte growth; Functionality maintenance

摘要：Bioartificial liver (BAL) comprising a functional bioreactor represents a promising treatment, bridging patients with acute liver failure (ALF) to liver transplantation. In this research, a packed-bed (PB) bioreactor by utilizing galactosylated poly(ethylene terephthalate) microfibrous carrier (PET-Gal) was fabricated and evaluated in vitro. Human induced hepatocytes (hiHeps) were dynamically seeded into a PET-Gal loaded bioreactor and cultured for 10 days. During perfusion culture, the effects of bioreactor scaffold on hepatocyte seeding, growth and functionality (albumin secretion and urea production) were determined, compared with that of control with nonmodified PET carrier. Afterwards, drug metabolism and detoxification ability of hiHeps were measured. Results demonstrated that the seeding rate and proliferation fold within PET-Gal loaded bioreactor were significantly higher than that of control, resulting in a cell density of (8.17 +/- 0.52) x 10(7) cells/cm(3). In contrast to flattened morphology observed in the control, hiHeps cultured in PET-Gal based bioreactor displayed as three-dimensional (3D) cell aggregates with close cell-matrix and cell-cell interactions and high cell viability, leading to higher albumin secretion and urea production. Besides, there were high levels of phase I drug metabolism and ammonia elimination for PET-Gal cultured cells. Therefore, the PET-Gal based PB bioreactor fabricated herein supports high-density hepatocyte growth and meanwhile preserves hepatic functionality, showing great potential in BAL systems application.