文献类型：期刊文献

英文题名：Enhanced production of flavonoids by methyl jasmonate elicitation in cell suspension culture of Hypericum perforatum

作者：Wang, Jing[1,2]; Qian, Jie[3]; Yao, Lingyun[1,2]; Lu, Yanhua[1,2]

机构：[1] State Key Laboratory of Bioreactor Engineering, Department of Bioengineering, East China University of Science and Technology, 130 Meilong Rd, Shanghai, 200237, China; [2] Shanghai Collaborative Innovation Center for Biomanufacturing Technology, 130 Meilong Road, Shanghai, 200237, China; [3] School of Life Science and Technology, Tongji University, Shanghai, 200092, China

年份：2015

卷号：2

期号：1

外文期刊名：Bioresources and Bioprocessing

收录：EI(收录号：20224513081488)

语种：英文

外文关键词：Amino acids - Cells - Flavonoids - Metabolites - Plants (botany)

摘要：Background: Flavonoids of Hypericum perforatum are important secondary metabolites which have been widely utilized in medicine for a range of purposes. The use of methyl jasmonate (MeJA) elicitation for the enhancement of flavonoid production in cell suspension culture of H. perforatum would be an efficient alternative method for the flavonoid production. Results: MeJA influenced the cells growth and flavonoid production. The optimal elicitation strategy was treatment of the cell cultures with 100 μmol/L MeJA on day 15, which resulted in the highest flavonoid production (280 mg/L) and 2.7 times of control cultures. The activities of catalase (CAT) were inhibited after MeJA treatment in the cell cultures, while the activities of phenylalanine ammonia lyase (PAL) increased, which led to the enhancement of flavonoid production. Conclusion: MeJA elicitation is a useful method for the enhancement of flavonoid production in cell suspension culture of H. perforatum. ? 2015 Wang et al.