文献类型：期刊文献

英文题名：Codon usage biases co-evolve with transcription termination machinery to suppress premature cleavage and polyadenylation

作者：Zhou, Zhipeng[1];Dang, Yunkun[2,3];Zhou, Mian[4];Yuan, Haiyan[1];Liu, Yi[1]

机构：[1]Univ Texas Southwestern Med Ctr Dallas, Dept Physiol, Dallas, TX 75390 USA;[2]Yunnan Univ, State Key Lab Conservat & Utilizat Bioresources Y, Kunming, Yunnan, Peoples R China;[3]Yunnan Univ, Sch Life Sci, Ctr Life Sci, Kunming, Yunnan, Peoples R China;[4]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China

年份：2018

卷号：7

外文期刊名：ELIFE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000428254600001)】；

基金：National Institute of General Medical Sciences R35GM118118 Yi LiuCancer Prevention and Research Institute of Texas RP160268 Yi LiuWelch Foundation I-1560 Yi Liu

语种：英文

摘要：Codon usage biases are found in all genomes and influence protein expression levels. The codon usage effect on protein expression was thought to be mainly due to its impact on translation. Here, we show that transcription termination is an important driving force for codon usage bias in eukaryotes. Using Neurospora crassa as a model organism, we demonstrated that introduction of rare codons results in premature transcription termination (PTT) within open reading frames and abolishment of full-length mRNA. PTT is a wide-spread phenomenon in Neurospora, and there is a strong negative correlation between codon usage bias and PTT events. Rare codons lead to the formation of putative poly(A) signals and PTT. A similar role for codon usage bias was also observed in mouse cells. Together, these results suggest that codon usage biases co-evolve with the transcription termination machinery to suppress premature termination of transcription and thus allow for optimal gene expression.