文献类型：期刊文献

英文题名：Induction of zonal-specific cellular morphology and matrix synthesis for biomimetic cartilage regeneration using hybrid scaffolds

作者：Owida, H. A.[1];Yang, R.[2];Cen, L.[2];Kuiper, N. J.[1,3];Yang, Y.[1]

机构：[1]Univ Keele, Inst Sci & Technol Med, Stoke On Trent ST4 7QB, Staffs, England;[2]East China Univ Sci & Technol, Shanghai Key Lab Multiphase Mat Chem Engn, Sch Chem Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Robert Jones & Agnes Hunt Orthopaed Hosp, Arthrit Res Ctr, Oswestry SY10 7AG, Shrops, England

年份：2018

卷号：15

期号：143

外文期刊名：JOURNAL OF THE ROYAL SOCIETY INTERFACE

收录：;EI(收录号：20182805539342);WOS:【SCI-EXPANDED(收录号:WOS:000437007600026)】；

基金：The authors are grateful for the British Council Newton Fund: PhD Placement grant for H.A.O. and the 'Open Funding Project of the State Key Laboratory of Bioreactor Engineering' funding from ECUST.

语种：英文

外文关键词：cartilage regeneration; zonal structure; hybrid scaffolds; chondrocyte morphology; extracellular matrix

摘要：Cartilage is anisotropic in nature and organized into distinct zones. Our goal was to develop zonal-specific three-dimensional hybrid scaffolds which could induce the generation of zonal-specific cellular morphology and extracellular matrix (ECM) composition. The superficial and middle zones comprised two layers of hyaluronic acid (HA) hydrogel which enveloped specifically orientated or randomly arranged polylactic acid nanofibre meshes. The deep zone comprised a HA hydrogel with multiple vertical channels. Primary bovine chondrocytes were seeded into the individual zonal scaffolds, cultured for 14 days and then the ECM was analysed. The aligned nanofibre mesh used in the superficial zone induced an elongated cell morphology, lower glycosaminoglycan (GAG) and collagen II production, and higher cell proliferation and collagen I production than the cells in the middle zone scaffold. Within the middle zone scaffold, which comprised a randomly orientated nanofibre mesh, the cells were clustered and expressed more collagen II. The deep zone scaffold induced the highest GAG production, the lowest cell proliferation and the lowest collagen I expression of the three zones. Assembling the three zones and stabilizing the arrangement with a HA hydrogel generated aligned, randomly aggregated and columnar cells in the superficial, middle and deep zones. This study presents a method to induce zonal-specific chondrocyte morphology and ECM production.