文献类型：期刊文献

英文题名：Deciphering Mechanism of Zhishi-Xiebai-Guizhi Decoction Against Hypoxia/Reoxygenation Injury in Cardiomyocytes by Cell Metabolomics: Regulation of Oxidative Stress and Energy Acquisition

作者：Lin, Chuhui[1]; Sang, Qingni[1]; Fu, Zhibo[1]; Yang, Shenglong[1]; Zhang, Min[2]; Zhang, Hongyang[1]; Wang, Yuerong[1]; Hu, Ping[1]

机构：[1] Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, China; [2] Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, China

年份：2022

外文期刊名：SSRN

收录：EI(收录号：20220378902)

语种：英文

外文关键词：Cell death - Diseases - Mergers and acquisitions - Metabolism - Metabolites - Mitochondria - Multivariant analysis

摘要：Myocardial ischemia/reperfusion (MI/R) injury is a life-threatening syndrome with high morbidity and mortality. Zhishi-Xiebai-Guizhi Decoction (ZSXBGZD) is a classic traditional Chinese medicine formula, used to treat cardiovascular diseases for centuries. However, its underlying medicinal mechanism has not been clearly elucidated, which hinders its widespread application. Here, the curative effects and therapeutic mechanism of ZSXBGZD against MI/R were addressed based on an integration of pharmaceutical evaluation and cellular metabolomics. First, a hypoxia/reoxygenation (H/R) model in H9c2 cells was employed to resemble MI/R and multiple pharmacological indicators were performed to assess the efficacy of ZSXBGZD. The results showed that ZSXBGZD possessed exceptional ability in attenuating cardiomyocyte injury, concerning oxidative stress, mitochondrial dysfunction, energy acquisition and cell apoptosis. Furthermore, a cell metabolomics approach based on HILIC and UPLC-QTOF-MS coupled with multivariate analysis was conducted to explore the metabolic regulation of ZSXBGZD. 38 differential polar metabolites related to H/R were uncovered, and 34 of them were reversed to normal state after the treatment of ZSXBGZD, revealing the perturbations of energy metabolism and amino acid metabolism. Moreover, formula decomposition justified the combination of single herbs to form ZSXBZGD and confirmed the pivotal status of Allii Macrostemonis Bulbus and Trichosanthis Fructus . ? 2022, The Authors. All rights reserved.