文献类型：期刊文献

英文题名：Comprehensive characterization of Stevia Rebaudiana using two-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography

作者：Fu, Qing[1,2];Guo, Zhimou[2];Zhang, Xiuli[2];Liu, Yanfang[2];Liang, Xinmiao[1,2]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Nat Med Liaoning Prov, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China

年份：2012

卷号：35

期号：14

起止页码：1821

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20123015283404);WOS:【SCI-EXPANDED(收录号:WOS:000306493700015)】；

基金：This work was supported by Project of Knowledge Innovation Program of Chinese Academy of Sciences (KSCX2-YW-R-214), Project of National Science Foundation of China (20805046), Natural Science Foundation of China (21005077), and foundation for Distinguished Young Scholars (Grant No. 20825518) from the National Natural Sciences Foundation of China.

语种：英文

外文关键词：Characterization; HILIC; RP; Stevia rebaudiana; Two-dimensional liquid chromatography (2D-LC)

摘要：Two-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography (2D-RPLC/HILIC) system was successfully applied for comprehensive characterization of steviol glycosides from Stevia rebaudiana. The experiments were performed in offline mode using an XCharge C18 column in first dimension and an XAmide column in second dimension. In first dimension, preliminary separation of Stevia aqueous extract was accomplished and 30 fractions were collected. Then fractions 120 were selected for further purification and 13 compounds with high purity were obtained in second dimension. Comprehensive characterization of these compounds was completed by determination of their retention time, accurate molecular weight, diagnostic fragmentation ions, and nuclear magnetic resonance spectroscopy. As a result, all nine known steviol glycosides, as well as other four steviol glycosides were fully purified. The result demonstrated that this procedure is an effective approach for the preparative separation and comprehensive characterization of steviol glycosides in Stevia. This 2D-RPLC/HILIC method will be a promising tool for the purification of low-abundance compounds from natural products.